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Physostigmine Salicylate

Name: Physostigmine Salicylate
Brand: TargetMol
SKU: T0130
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0130Cas No. 57-64-7

Physostigmine, a parasympathomimetic alkaloid, is a reversible cholinesterase inhibitor. It extracts from the Calabar bean.

Physostigmine Salicylate

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Purity: 99.86%

Catalog No. T0130Cas No. 57-64-7

Physostigmine, a parasympathomimetic alkaloid, is a reversible cholinesterase inhibitor. It extracts from the Calabar bean.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$33	In Stock	In Stock
5 mg	$75	In Stock	In Stock
10 mg	$103	In Stock	In Stock
25 mg	$180	In Stock	In Stock
50 mg	$253	In Stock	In Stock
100 mg	$345	-	In Stock
1 mL x 10 mM (in DMSO)	$82	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Batch Information

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Purity:99.86%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Physostigmine Salicylate AI Summary

Physostigmine Salicylate exhibits strong bioactivity and potent inhibition properties across multiple targets. It inhibits human erythrocyte Acetylcholinesterase (AChE) with an IC50 value of 28.0 nM and human plasma Butyrylcholinesterase (BChE) with an IC50 value of 16.0 nM, showing a moderate selectivity value of 2.0. Additionally, it inhibits Torpedo california AChE with an IC50 of 21.3 nM, displaying concentration-dependent inhibition ranging from 24.71% to 64.3% at different concentrations (7.56 nM to 30.3 nM). The compound also demonstrates reversible mixed-type inhibition of electric eel acetylcholinesterase with a Ki value of 210.0 nM. Beyond cholinesterase inhibition, Physostigmine Salicylate shows various bioactivities, including: 1. Potent inhibition of Human Jumonji Domain Containing 2E (JMJD2E). 2. Enhancement of SMN2 splice variant expression. 3. Agonistic and antagonistic properties on the Hypoxia Response Element Signaling Pathway. 4. Inhibition of Aldehyde Dehydrogenase 1 (ALDH1A1) and 15-Hydroxyprostaglandin Dehydrogenase (HPGD). 5. Inhibition of Chronic Active B-Cell Receptor Signaling and the malarial parasite plastid with delayed death activity. 6. Substrate-like properties for Mammalian Selenoprotein Thioredoxin Reductase 1 (TrxR1). 7. Inducing DNA re-replication in MCF 10a normal breast cells and inhibiting Glutaminase (GLS). 8. Inducing synthetic lethality in tumor cells producing 2HG, specifically in the HT-1080-NT fibrosarcoma cell line. Furthermore, Physostigmine Salicylate inhibits sodium fluorescein uptake in both OATP1B1 and OATP1B3-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 µM, with inhibition percentages of 115.97% and 110.77%, respectively, suggesting a high affinity for these transporters..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description

Physostigmine, a parasympathomimetic alkaloid, is a reversible cholinesterase inhibitor. It extracts from the Calabar bean.

Chemical Properties

Molecular Weight	413.48
Formula	C₂₂H₂₇N₃O₅
Cas No.	57-64-7
Smiles	OC(=O)c1ccccc1O.[H][C@]12N(C)CC[C@@]1(C)c1cc(OC(=O)NC)ccc1N2C
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 125 mg/mL (302.31 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.84 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4185 mL	12.0925 mL	24.1850 mL	120.9248 mL
5 mM	0.4837 mL	2.4185 mL	4.8370 mL	24.1850 mL
10 mM	0.2418 mL	1.2092 mL	2.4185 mL	12.0925 mL
20 mM	0.1209 mL	0.6046 mL	1.2092 mL	6.0462 mL
50 mM	0.0484 mL	0.2418 mL	0.4837 mL	2.4185 mL
100 mM	0.0242 mL	0.1209 mL	0.2418 mL	1.2092 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc