Shopping Cart

Your shopping cart is currently empty

Physostigmine (Synonyms: Eserine)

Name: Physostigmine
Brand: TargetMol
SKU: T21438
Price: 189 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T21438 Copy Product Info

🥰Excellent

Physostigmine is an alkaloid extracted from the poison bean (Physostigma venenosum) and acts as a reversible acetylcholinesterase (AChE) inhibitor. Physostigmine inhibits the action of acetylcholinesterase, increasing acetylcholine levels, and thus serves as an antidote for anticholinergic poisoning. Physostigmine crosses the blood-brain barrier and stimulates central cholinergic neurotransmission. Physostigmine can reverse memory deficits in transgenic mice with Alzheimer's disease.

Physostigmine

Copy Product Info

🥰Excellent

Catalog No. T21438

Synonyms Eserine

Cas No. 57-47-6

Pack Size	Price	USA Stock	Global Stock
1 mg	$189	-	In Stock
5 mg	$475	-	In Stock
10 mg	$682	-	In Stock
25 mg	$987	-	In Stock
50 mg	$1,380	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Appearance:Solid

Color:Yellow

Resource Download

COA HNMR

Product Introduction

Bioactivity

Description	Physostigmine is an alkaloid extracted from the poison bean (Physostigma venenosum) and acts as a reversible acetylcholinesterase (AChE) inhibitor. Physostigmine inhibits the action of acetylcholinesterase, increasing acetylcholine levels, and thus serves as an antidote for anticholinergic poisoning. Physostigmine crosses the blood-brain barrier and stimulates central cholinergic neurotransmission. Physostigmine can reverse memory deficits in transgenic mice with Alzheimer's disease.
In vitro	Methods: Tissue homogenates were prepared from the CA3-CA1 region of the hippocampus. After perfusion with 10 μM Physostigmine for 20 minutes, acetylcholinesterase (AChE) activity was measured using a colorimetric assay immediately after perfusion, 20 minutes after elution, and 1 hour after elution. Results: AChE activity increased after 20 min of Physostigmine perfusion, returned to baseline after 20 min of elution, and showed a compensatory increase after 1 h of elution. [1] Methods: Primary rat cortical neurons were treated with Physostigmine (0.01, 0.1, 1.0, 10, 100 nM) for 24 h. Live-cell imaging (1 frame every 2 seconds for a total of 3 minutes) was performed to generate a kymograph, which was analyzed using KymoAnalyzer. Results: None of the Physostigmine concentrations had a significant effect on axonal transport velocity (anterior direction); conversely, the highest concentration (100 nM) significantly increased retrograde transport velocity.[2]
In vivo	Methods: To investigate the effects of Physostigmine, adult male C57BL/6J mice were administered Physostigmine (0.25 mg/kg) via intraperitoneal injection. Twenty minutes after the initial injection, ketamine (5 mg/kg) was administered intraperitoneally. The forced swim test was conducted 50 minutes later, and the immobility time within the first 4 minutes was recorded. Results: Physostigmine significantly increased the immobility time; ketamine significantly reduced the increase in immobility time induced by Physostigmine, indicating that Physostigmine impairs neurotransmission. [1]
Synonyms	Eserine

Chemical Properties

Molecular Weight	275.35
Formula	C₁₅H₂₁N₃O₂
Cas No.	57-47-6
Smiles	C[C@]12C=3C(N(C)[C@]1(N(C)CC2)[H])=CC=C(OC(NC)=O)C3
Relative Density.	1.166g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (145.27 mM), Sonication is recommended.

H2O: Insoluble

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.6317 mL	18.1587 mL	36.3174 mL	181.5871 mL
5 mM	0.7263 mL	3.6317 mL	7.2635 mL	36.3174 mL
10 mM	0.3632 mL	1.8159 mL	3.6317 mL	18.1587 mL
20 mM	0.1816 mL	0.9079 mL	1.8159 mL	9.0794 mL
50 mM	0.0726 mL	0.3632 mL	0.7263 mL	3.6317 mL
100 mM	0.0363 mL	0.1816 mL	0.3632 mL	1.8159 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Citations

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc