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Osthole

Name: Osthole
Brand: TargetMol
SKU: T2848
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2848Cas No. 484-12-8

Alias Ostol, Osthol, NSC 31868

Osthole (Osthol), a potential inhibitor of histamine H1 receptor, has been shown to stimulate osteoblast proliferation and differentiation.

Osthole

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Purity: 99.96%

Catalog No. T2848Alias Ostol, Osthol, NSC 31868Cas No. 484-12-8

Osthole (Osthol), a potential inhibitor of histamine H1 receptor, has been shown to stimulate osteoblast proliferation and differentiation.

Pack Size	Price	USA Warehouse	Global Warehouse
200 mg	$30	In Stock	In Stock
500 mg	$60	In Stock	In Stock
1 g	$97	In Stock	In Stock
2 g	$148	-	In Stock
1 mL x 10 mM (in DMSO)	$36	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.96%

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	Osthole (Osthol), a potential inhibitor of histamine H1 receptor, has been shown to stimulate osteoblast proliferation and differentiation.
In vitro	The LD50 values of Osthole in various animals have been determined as follows: in rats, it exceeds 125 mg/kg when administered intravenously (i.v.); in rabbits, it is over 35 mg/kg (i.v.); and in dogs, it surpasses 150 mg/kg (i.v.).
In vivo	Osthol, when introduced to pre-washed rabbit platelets, inhibits platelet aggregation and ATP release induced by ADP, arachidonic acid, platelet-activating factor (PAF), collagen, ionophore A23187, and thrombin. In human osteoblasts, osthol promotes cell differentiation through the bone morphogenetic protein-2 / p38 and extracellular signal-regulated kinase 1/2 pathways. Additionally, in cell cultures, osthol suppresses the secretion of the hepatitis B virus by enhancing the glycosylation of HBsAg.
Cell Research	Osthole is dissolved in 96% ethyl alcohol and then sterilized through a 0.22 μg/mL filter and stored at 4°C as stock solutions for later dilution[1]. Peripheral blood samples are collected from participants between 7.00 and 9.00 a.m. on the first study day and these are concentrated in grouping tubes with K3EDTA. Fresh PBMCs are then prepared. Isolated cells are seeded on 24-well plates at 1×106 per well with RPMI-1640 and supplemented with 1% heat inactivated human AB serum, 1% gentamicin and 0.25% PHA. Active reagents are added to each well after 24 h and pure medium formed the control for each substance. Cells are then harvested after a further three days[1].
Synonyms	Ostol, Osthol, NSC 31868

Chemical Properties

Molecular Weight	244.29
Formula	C₁₅H₁₆O₃
Cas No.	484-12-8
Smiles	COC1=C(C\C=C(/C)C)C2=C(C=CC(=O)O2)C=C1
Relative Density.	1.126 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 46 mg/mL (188.3 mM), Sonication is recommended.

DMSO: 55 mg/mL (225.14 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (8.19 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.0935 mL	20.4675 mL	40.9350 mL	204.6748 mL
5 mM	0.8187 mL	4.0935 mL	8.1870 mL	40.9350 mL
10 mM	0.4093 mL	2.0467 mL	4.0935 mL	20.4675 mL
20 mM	0.2047 mL	1.0234 mL	2.0467 mL	10.2337 mL
50 mM	0.0819 mL	0.4093 mL	0.8187 mL	4.0935 mL
100 mM	0.0409 mL	0.2047 mL	0.4093 mL	2.0467 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc