Home Tools

L48H37

Catalog No. T40423 CAS 343307-76-6

L48H37 is a chemically stable analog of Curcumin. It exhibits potent inhibitory properties against myeloid differentiation protein 2 (MD2), acting as a specific inhibitor. Its mechanism involves inhibiting the interaction and signaling transduction of LPS-TLR4/MD2. L48H37 is primarily utilized in sepsis and lung injury research [1].

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

L48H37, CAS 343307-76-6

Pack Size	Availability	Price/USD
2 mg	In stock	$ 39.00
5 mg	In stock	$ 64.00
10 mg	In stock	$ 97.00
25 mg	In stock	$ 197.00
50 mg	In stock	$ 313.00
100 mg	In stock	$ 513.00
500 mg	In stock	$ 1,090.00
1 mL * 10 mM (in DMSO)	In stock	$ 79.00

Bulk Inquiry

Select Batch

Biological Description

Chemical Properties

Storage & Solubility Information

Description	L48H37 is a chemically stable analog of Curcumin. It exhibits potent inhibitory properties against myeloid differentiation protein 2 (MD2), acting as a specific inhibitor. Its mechanism involves inhibiting the interaction and signaling transduction of LPS-TLR4/MD2. L48H37 is primarily utilized in sepsis and lung injury research [1].
In vitro	L48H37 effectively suppresses LPS-induced inflammation in mouse macrophages, particularly diminishing TNF-α and IL-6 production and gene expression [1]. It also demonstrates noteworthy anticancer activity by reducing the viability of A549 and H460 lung cancer cells with IC50 values of 5.3 μM and 2.3 μM, respectively, outperforming curcumin. Importantly, its cytotoxicity towards normal human lung epithelial cells (BEAS-2B) remains low, with an IC50 of 21 μM [2]. Moreover, L48H37 at concentrations of 1, 2, or 4 μM over 16 hours decreases p-Cdc2 and Cdc2 levels, increases p53, enhances cleaved poly (ADP-ribosyl) polymerase (PARP) levels, and reduces anti-apoptotic protein Bcl-2 expression in both H460 and A549 cells, indicating apoptosis induction. Additionally, at 4 μM for 16 hours, L48H37 dose-dependently elevates intracellular ROS levels, as evidenced by increased DCF levels in these cells [2]. Cell viability assays and Western Blot analysis confirm its concentration-dependent efficacy in inhibiting lung cancer cell growth and modulating key proteins involved in cell cycle and apoptosis [2].
In vivo	L48H37, administered via intraperitoneal injection at doses of 5 mg or 10 mg/kg once daily for 11 days, effectively inhibits the growth of H460 xenograft tumors and demonstrates significant anti-tumor effects in mice. This study utilized 5-week-old athymic BALB/cA nu/nu female mice weighing 18-22 g. The treatment resulted in reduced tumor wet weights compared to the control group, decreased levels of phosphorylated STAT3, and increased levels of phosphorylated EIF2α and ATF4, indicating a potent anti-tumor response without causing significant structural alterations in the mice.

Molecular Weight	483.55
Formula	C27H33NO7
CAS No.	343307-76-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 45 mg/mL (93.06 mM), Sonication is recommended.

References and Literature

1. Yi Wang, et al. Curcumin Analog L48H37 Prevents Lipopolysaccharide-Induced TLR4 Signaling Pathway Activation and Sepsis via Targeting MD2. J Pharmacol Exp Ther. 2015 Jun;353(3):539-50 2. Chen Feng, et al. Curcumin analog L48H37 induces apoptosis through ROS-mediated endoplasmic reticulum stress and STAT3 pathways in human lung cancer cells. Mol Carcinog. 2017 Jul;56(7):1765-1777.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Membrane Protein-targeted Compound Library Bioactive Compounds Library Max Bioactive Compound Library Pyroptosis Compound Library PPI Inhibitor Library Immunology/Inflammation Compound Library

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

L48H37 343307-76-6 inhibit Inflammation lung injury TLR4/MD2 Asthma IL-6 Toll-like Receptor (TLR) Sepsis Curcumin Inhibitor TNF-α L-48H37 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.