Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Disulfiram

Disulfiram
Disulfiram (TETD) is an inhibitor of the acetaldehyde dehydrogenase ALDH, inhibiting hALDH1 and hALDH2 (IC50=0.15/1.45 μM) with specificity. Disulfiram is acutely sensitized to alcohol and also inhibits GSDMD pore formation.
Catalog No. T0054Cas No. 97-77-8
Select Batch
Purity:99.79%
Contact us for more batch information

Resource Download

Disulfiram

Catalog No. T0054Cas No. 97-77-8

Disulfiram (TETD) is an inhibitor of the acetaldehyde dehydrogenase ALDH, inhibiting hALDH1 and hALDH2 (IC50=0.15/1.45 μM) with specificity. Disulfiram is acutely sensitized to alcohol and also inhibits GSDMD pore formation.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
1 g$50In Stock
1 mL x 10 mM (in DMSO)$50In Stock
Bulk & Custom
Add to Cart
Questions
View More

Related Compound Libraries of "Disulfiram"

Product Introduction

Bioactivity
Description
Disulfiram (TETD) is an inhibitor of the acetaldehyde dehydrogenase ALDH, inhibiting hALDH1 and hALDH2 (IC50=0.15/1.45 μM) with specificity. Disulfiram is acutely sensitized to alcohol and also inhibits GSDMD pore formation.
In vitro
Disulfiram inhibits the P-glycoprotein efflux pump, suppresses the transcription factor NF-κB (nuclear factor-κB), reduces angiogenesis, and inhibits tumor growth in mice. Its antitumor activity is associated with in vivo protease inhibition. Disulfiram also induces apoptosis. In severe combined immunodeficient mice with melanoma xenografts, it impedes growth and angiogenesis, an effect that is enhanced by zinc supplementation.
In vivo
Disulfiram, clinically employed as an anti-alcoholism agent, potently inhibits both constitutive and 5-FU-induced NF-κB activity in a dose-dependent manner. In the DLD-1 and RKO (WT) cell lines, Disulfiram significantly augments the apoptotic effects of 5-FU and synergistically enhances its cytotoxicity. In melanoma cells, co-treatment with copper or zinc ions reduces cyclin A expression compared to Disulfiram alone and inhibits proliferation in vitro. Moreover, Disulfiram decreases viable cell count, with copper chloride addition markedly intensifying DSF-induced cell death.
Cell Research
The effect of disulfiram (0.15-5.0 μM) or sodium diethyldithiocarbamate (1.0 μM) on proliferation of malignant cell lines is studied in cultures stimulated with 10% FBS. Cell numbers are quantitated 24 to 72 hours later, as outlined below. In some experiments, disulfiram is added immediately after cells are plated. In other experiments, cells are plated and allowed to grow for 24 to 72 hours before fresh medium with disulfiram is added and cell numbers are assayed 24 to 72 hours later. Synergy is studied between disulfiram and?N,N′-bis(2-chloroethyl-N-nitrosourea (carmustine, 1.0-1,000 μM) or cisplatin (0.1-100 μg/mL) added to medium. The effect of metal ions on disulfiram is studied with 0.2 to 10 μM Cu2+?(provided as CuSO4), Zn2+?(as ZnCl2), Ag+?(as silver lactate), or Au3+?(as HAuCl4·3Water) ions added to growth medium, buffered to physiologic pH. To provide a biologically relevant source of copper, medium is supplemented with human ceruloplasmin at doses replicating low and high normal adult serum concentrations (250 and 500 mg/mL).
AliasTetraethylthiuram disulfide, NSC 190940, TETD
Chemical Properties
Molecular Weight296.54
FormulaC10H20N2S4
Cas No.97-77-8
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 20 mg/mL (67.44 mM), Sonication is recommended.
Ethanol: 29.7 mg/mL (100 mM)
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2.97 mg/mL (10.02 mM), Working solution is recommended to be prepared and used immediately.
Solution Preparation Table
10% DMSO+40% PEG300+5% Tween 80+45% Saline/DMSO/Ethanol
1mg5mg10mg50mg
1 mM3.3722 mL16.8611 mL33.7223 mL168.6113 mL
5 mM0.6744 mL3.3722 mL6.7445 mL33.7223 mL
10 mM0.3372 mL1.6861 mL3.3722 mL16.8611 mL
DMSO/Ethanol
1mg5mg10mg50mg
20 mM0.1686 mL0.8431 mL1.6861 mL8.4306 mL
50 mM0.0674 mL0.3372 mL0.6744 mL3.3722 mL
Ethanol
1mg5mg10mg50mg
100 mM0.0337 mL0.1686 mL0.3372 mL1.6861 mL

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
%Tween 80
%ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy Disulfiram | purchase Disulfiram | Disulfiram cost | order Disulfiram | Disulfiram chemical structure | Disulfiram in vivo | Disulfiram in vitro | Disulfiram formula | Disulfiram molecular weight