IL-4 Protein, Mouse, Recombinant

Name: IL-4 Protein, Mouse, Recombinant
Brand: TargetMol
SKU: TMPY-02558
Price: 125 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: interleukin 4, Il-4, BSF-1) Copy Product Info

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Synonyms: interleukin 4, Il-4, BSF-1

Catalog No. TMPY-02558 Copy Product Info

Purity: 98.7%

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IL-4 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 13.4 kDa and the accession number is P07750.

Pack Size	Price	USA Stock	Global Stock
5 µg	$125	In Stock	In Stock
10 µg	$163	-	In Stock
20 µg	$213	In Stock	In Stock
50 µg	$297	-	In Stock
100 µg	$405	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.7%

Appearance:Lyophilized powder

Color:White

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COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is typically 0.4-4 ng/mL.
Description	IL-4 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 13.4 kDa and the accession number is P07750.
Species	Mouse
Expression System	E. coli
Tag	Tag Free
Accession Number	P07750
Construction	A DNA sequence encoding the mouse IL4 (NP_067258.1) (His23-Ser140) was expressed with a N-terminal Met. Predicted N terminal: Met
Protein Purity	> 90% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.1 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	interleukin 4, Il-4, BSF-1
Research Background	Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

13.4 kDa (predicted); 14 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.