Anti-Dopamine beta-Hydroxylase Polyclonal Antibody

Anti-Dopamine beta-Hydroxylase Polyclonal Antibody is a Rabbit antibody targeting Dopamine beta-Hydroxylase. Anti-Dopamine beta-Hydroxylase Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat (predicted:Mouse)

1. Sample:
Jurkat (Human) Cell Lysate at 40 μg
Primary: Anti-Dopamine beta Hydroxylase (TMAB-00562) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DOPO) Polyclonal Antibody, Unconjugated (TMAB-00562) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Blank control (black line): SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Dopamine beta Hydroxylase antibody (TMAB-00562)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human DBH

Human

DBH

Dopamine beta-hydroxylase

Hormone biosynthesis,Hormone biosynthesis,Dopamine

Conversion of dopamine to noradrenaline.