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Caspase-3 Protein, Human, Recombinant (aa 29-277, His) is expressed in E. coli. The accession number is P42574.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 μg | $77 | 7-10 days | 7-10 days | |
| 10 μg | $126 | 7-10 days | 7-10 days | |
| 20 μg | $198 | 7-10 days | 7-10 days | |
| 50 μg | $397 | 7-10 days | 7-10 days | |
| 100 μg | $796 | 7-10 days | 7-10 days | |
| 200 μg | $1,200 | 7-10 days | 7-10 days | |
| 500 μg | $2,590 | 7-10 days | 7-10 days | |
| 1 mg | $5,280 | 7-10 days | 7-10 days |
| Biological Activity | Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC. The specific activity is > 5000 pmoles/min/μg. |
| Description | Caspase-3 Protein, Human, Recombinant (aa 29-277, His) is expressed in E. coli. The accession number is P42574. |
| Species | Human |
| Expression System | E. coli |
| Tag | C-His |
| Accession Number | P42574 |
| Synonyms | Yama,SCA-1,CPP32B,CPP32,apopain |
| Construction | A DNA sequence encoding the mature form of human caspase3 (P42574) (Ser29-His277) was expressed with a polyhistidine tag at the C-terminus. |
| Protein Purity | > 95% as determined by SDS-PAGE |
| Molecular Weight | 29.9 kDa (predicted); 17 and 15 kDa (reducing contition) |
| Endotoxin | Please contact us for more information. |
| Formulation | Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Please refer to the lot-specific COA. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, Lyophilized powders are shipping with blue ice. |
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