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Anti-TNF alpha Antibody (4V549)
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Catalog No. TMAB-01869
Anti-TNF alpha Antibody (4V549) is a Mouse antibody targeting TNF alpha. Anti-TNF alpha Antibody (4V549) can be used in WB.
Anti-TNF alpha Antibody (4V549)
Anti-TNF alpha Antibody (4V549)
😃Good
Catalog No. TMAB-01869
Anti-TNF alpha Antibody (4V549) is a Mouse antibody targeting TNF alpha. Anti-TNF alpha Antibody (4V549) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-TNF alpha Antibody (4V549) is a Mouse antibody targeting TNF alpha. Anti-TNF alpha Antibody (4V549) can be used in WB. |
| Ig Type | IgG1 |
| Clone | 4V549 |
| Reactivity | Human |
| Verified Activity | THP-1 (H) cells were treated with or without LPS (100 ng/ml) for 16 h and Brefeldin A (5 µg/ml) for 4 h, 25 μg total protein per Lane of cell lysates probed with TNF alpha monoclonal antibody, unconjugated (TMAB-01869) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein. Tumor necrosis factor, soluble form: Secreted. C-domain 1: Secreted. C-domain 2: Secreted. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020] |
| Immunogen | Recombinant Protein: human TNF alpha 77-233aa |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | TNF,Inflammatory mediators,TNF Superfamily,Metabolic disorders,SARS Coronavirus,TNF |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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