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Angiotensin II human

Name: Angiotensin II human
Brand: TargetMol
SKU: T7040
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7040Cas No. 4474-91-3

Alias Hypertensin II, DRVYIHPF, Angiotensin II, Ang II

Angiotensin II is a major biologically active vasoconstrictor peptide that regulates blood pressure by binding to AT1R and AT2R receptors. It stimulates the sympathetic nervous system, promotes aldosterone synthesis, and enhances renal function. Additionally, it induces vascular smooth muscle cell proliferation and increases collagen synthesis, leading to vascular and myocardial hypertrophy and fibrosis. It also promotes apoptosis and endothelial capillary formation. Angiotensin II is commonly used to induce hypertension and cardiac hypertrophy animal models.

Angiotensin II human

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Purity: 99.97%

Catalog No. T7040Alias Hypertensin II, DRVYIHPF, Angiotensin II, Ang IICas No. 4474-91-3

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$37	In Stock	In Stock
10 mg	$59	In Stock	In Stock
25 mg	$123	In Stock	In Stock
50 mg	$225	In Stock	-
100 mg	$321	In Stock	-
500 mg	$779	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.97%

Color:White

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Product Introduction

Bioactivity

Description	Angiotensin II is a major biologically active vasoconstrictor peptide that regulates blood pressure by binding to AT1R and AT2R receptors. It stimulates the sympathetic nervous system, promotes aldosterone synthesis, and enhances renal function. Additionally, it induces vascular smooth muscle cell proliferation and increases collagen synthesis, leading to vascular and myocardial hypertrophy and fibrosis. It also promotes apoptosis and endothelial capillary formation. Angiotensin II is commonly used to induce hypertension and cardiac hypertrophy animal models.
Targets&IC50	CHO cells viability:0.52 nM (EC50), CHO cells viability:1.5 nM
In vitro	METHODS: Human hepatocellular carcinoma cells (HCC) HepG-2, SMMC-7721, and MHCC97-H were treated with Angiotensin II (1-1000 nM) for 24-72 h. The cell viability was determined using MTT. RESULTS: Angiotensin II induced HCC cell lines to show higher growth in a time- and concentration-dependent manner. [1] METHODS: Neonatal rat cardiomyocytes were treated with Angiotensin II (1 μmol/L) for 5 min-48 h. HMGB1 and IL-6 expression levels were measured by ELISA and RT-qPCR. RESULTS: Angiotensin II enhanced the expression levels of HMGB1 and IL-6 in cardiomyocytes. [2]
In vivo	METHODS: To determine insulin action in a human hypertensive mouse model, Angiotensin II (1.1 mg/kg in 0.9% saline) was administered to C57Bl/6J mice using an osmotic minipump for two to four weeks. RESULTS: Blood pressure increased after Angiotensin II treatment. The increase in serum insulin was greater in Angiotensin II-treated mice after glucose administration. Long-term Angiotensin II treatment for four weeks enhanced glucose-stimulated insulin secretion in mice. [3] METHODS: To analyze genotoxic effects in vivo, Angiotensin II (60 ng/kg/min-1 μg/kg/min) was administered using an osmotic minipump to C57Bl/6J mice for four weeks. RESULTS: Angiotensin II increased SBP up to 38 mmHg over control and adversely affected renal function in mice. In the heart, a significant increase in reactive oxygen species formation and double-strand breaks were detected at the highest administered dose. In the kidney, a dose-dependent increase in superoxide formation, double-strand breaks and DNA base modification mutations were observed. [4]
Disease Modeling Protocol	Hypertension model Modeling Mechanism： Angiotensin II human (Ang II) enhances vascular receptor affinity by binding to specific receptors on the surface of vascular smooth muscle cells, promoting vasoconstriction and increasing peripheral resistance. At the same time, it activates the sympathetic nervous system and regulates sodium balance (high sodium intake can further enhance receptor sensitivity), inducing persistent hypertension and mimicking the pathological process of clinical renin-angiotensin system activation related to hypertension. Related Products： Angiotensin II human (T7040) Modeling Method： Experimental Subject：Rats, Sprague-Dawley (SD), Male, Body weight 150–250 g Dosage and Administration Route：① Core modelling: Angiotensin II human, Dose adjusted as required for experiment (for receptor blockade studies, exogenously administered at 50 ng per dose to validate blockade effect), Dissolved in sterile physiological saline, Intravenous injection (i.v.); ② Dietary intervention: - High-sodium group: Standard diet+0.9% saline drinking water; - Normal sodium group: Standard diet (containing 0.42% sodium)+conventional drinking water; - Low-sodium group: Sodium-free diet+conventional drinking water; ③ Control treatment: Control group administered equal volume saline solution, with all other conditions identical; ④ Combined Preparation of Hypertensive Models: - Bilateral renal artery clamping (Goldblatt): Silver clamp applied to left renal artery, contralateral kidney preserved; - Unilateral nephrectomy (Goldblatt): Left renal artery ligated with silver clip, contralateral kidney removed; - DOCA hypertension: Subcutaneous implantation of 25 mg DOCA pellets (every 4 weeks)+high-sodium diet; Dosing Frequency and Duration Model：Single administration; dietary intervention commenced after 6 weeks Validation： 1. Blood Pressure Indicators: - Baseline Blood Pressure: Normal rat systolic blood pressure is 120-130 mmHg; after modeling, it reaches 175-195 mmHg in the two-kidney-one-clip type and over 210 mmHg in the one-kidney-one-clip type; spontaneously hypertensive rats reach 195-200 mmHg. - Receptor Blocking Verification: After injection of Ang II antibody, blood pressure can decrease by 20-50 mmHg (significant in the two-kidney-one-clip type, no significant decrease in the one-kidney-one-clip type). 2. Receptor Affinity: The high-sodium intake group requires 8 times more Ang II antibody than the low-sodium group to block the vascular response, proving that receptor sensitivity is positively correlated with sodium intake. 3. Metabolic Indicators: Plasma Ang II levels (approximately 150 pg/ml in the low-sodium group, < 20 pg/ml in the high-sodium group) show an inverse relationship with receptor affinity. 4. Specificity Verification: The antibody only blocks the pressor effect of Ang II and does not affect the blood pressure response to norepinephrine. Precautions： References：Brunner HR,et,al. Angiotensin II vascular receptors: their avidity in relationship to sodium balance, the autonomic nervous system, and hypertension. J Clin Invest. 1972 Jan;51(1):58-67.
Animal Research	(129×C57BL/6) F1 mice, which lack AT1A receptors Angiotensin II used, are fed 10 gm/day gelled 0.25% NaCl diet that contains all nutrients and water. After 28 days of Angiotensin II infusion, hearts are harvested, weighed fixed in formalin, sectioned, and stained with Masson trichrome.
Synonyms	Hypertensin II, DRVYIHPF, Angiotensin II, Ang II

Chemical Properties

Molecular Weight	1046.18
Formula	C₅₀H₇₁N₁₃O₁₂
Cas No.	4474-91-3
Smiles	CCC(C)C(NC(=O)C(Cc1ccc(O)cc1)NC(=O)C(NC(=O)C(CCCN=C(N)N)NC(=O)C(N)CC(O)=O)C(C)C)C(=O)NC(Cc1cnc[nH]1)C(=O)N1CCCC1C(=O)NC(Cc1ccccc1)C(O)=O
Relative Density.	1.43g/cm3
Sequence	Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
Sequence Short	DRVYIHPF

Storage & Solubility Information

Storage

keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 10.52 mg/mL (10.06 mM), Sonication is recommended.

DMSO: < 1 mg/mL (insoluble)

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	0.9559 mL	4.7793 mL	9.5586 mL	47.7929 mL
5 mM	0.1912 mL	0.9559 mL	1.9117 mL	9.5586 mL
10 mM	0.0956 mL	0.4779 mL	0.9559 mL	4.7793 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

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10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

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Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

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All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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