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Amiloride hydrochloride

Name: Amiloride hydrochloride
Brand: TargetMol
SKU: T0175
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0175Cas No. 2016-88-8

Alias MK-870 hydrochloride, Amiloride HCl

Amiloride hydrochloride (Amiloride HCl) is an effective sodium channel blocker of epithelial.

Amiloride hydrochloride

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Purity: 99.95%

Catalog No. T0175Alias MK-870 hydrochloride, Amiloride HClCas No. 2016-88-8

Amiloride hydrochloride (Amiloride HCl) is an effective sodium channel blocker of epithelial.

Pack Size	Price	USA Warehouse	Global Warehouse
50 mg	$37	In Stock	In Stock
100 mg	$53	In Stock	In Stock
500 mg	$97	-	In Stock
1 mL x 10 mM (in DMSO)	$39	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Purity:99.95%

Color:White to Yellow

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Amiloride hydrochloride (Amiloride HCl) is an effective sodium channel blocker of epithelial.
Targets&IC50	uPA:7 μM(Ki)
In vitro	In salt-sensitive hypertensive animals, Amiloride antagonizes or blocks the action of aldosterone in cells, as well as within vascular and renal tissues. Daily subcutaneous injections of 1 mg/kg Amiloride in DOCA-salt hypertensive rats reversed the initial increase in collagen deposition. In stroke-prone spontaneously hypertensive rats, Amiloride administration via saline drinking water delayed the onset of proteinuria and improved histological scores in brain and kidney tissues compared to controls.
In vivo	Upon increased perfusion pressure, 1 μM Amiloride and 30 nM Benzamil inhibit myogenic responses by blocking the activity of ENaC proteins, thereby suppressing vasoconstriction. In vascular smooth muscle cells, Amiloride completely inhibits sodium influx. Amiloride is a relatively selective inhibitor of epithelial sodium channels (ENaC) with an IC 50 of 0.1 μM to 0.5 μM. Although less effective as a Na+/H+ exchanger (NHE) inhibitor, Amiloride's IC 50 values range from as low as 3 μM at low external sodium concentrations to 1 mM at high external sodium concentrations. Furthermore, Amiloride exerts a weak inhibitory effect on Na+/Ca2+ exchange (NCX) with an IC 50 of 1 mM.
Synonyms	MK-870 hydrochloride, Amiloride HCl

Chemical Properties

Molecular Weight	266.09
Formula	C₆H₉Cl₂N₇O
Cas No.	2016-88-8
Smiles	Cl.NC(=N)NC(=O)c1nc(Cl)c(N)nc1N
Relative Density.	2.11 g/cm3

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 1 mg/mL (insoluble)

DMSO: 50 mg/mL (187.91 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.52 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7581 mL	18.7906 mL	37.5813 mL	187.9063 mL
5 mM	0.7516 mL	3.7581 mL	7.5163 mL	37.5813 mL
10 mM	0.3758 mL	1.8791 mL	3.7581 mL	18.7906 mL
20 mM	0.1879 mL	0.9395 mL	1.8791 mL	9.3953 mL
50 mM	0.0752 mL	0.3758 mL	0.7516 mL	3.7581 mL
100 mM	0.0376 mL	0.1879 mL	0.3758 mL	1.8791 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc