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A66

Name: A66
Brand: TargetMol
SKU: T2418
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2418Cas No. 1166227-08-2

A66 is a specific and effective p110α inhibitor(IC50=32 nM).

A66

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Purity: 99.51%

Catalog No. T2418Cas No. 1166227-08-2

A66 is a specific and effective p110α inhibitor(IC50=32 nM).

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$30	In Stock	In Stock
5 mg	$46	In Stock	In Stock
10 mg	$76	In Stock	In Stock
25 mg	$147	In Stock	In Stock
50 mg	$259	In Stock	In Stock
100 mg	$383	In Stock	In Stock
200 mg	$538	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$51	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.51%

Color:White

Resource Download

COA HPLC HNMR LCMS

Product Introduction

Bioactivity

Description	A66 is a specific and effective p110α inhibitor(IC50=32 nM).
Targets&IC50	p110α-E545K:30 nM, p110α-H1047R:43 nM, p110γ:3480 nM, p110α:32 nM, PI4Kβ:236 nM, PI3KC2β:462 nM
In vitro	In male CD1 mice, A66 (10 mg/kg) increased glucose production during the pyruvate tolerance test. In SK-OV-3 tumor tissues, A66 (100 mg/kg) effectively reduced the phosphorylation of Akt/PKB and p70 S6 kinase, inhibiting tumor cell growth.
In vivo	A66 significantly inhibits the oncogenic forms of p110α, such as p110αE545K (IC50=30 nM) and p110αH1047R (IC50=43 nM), effectively reducing their activity.
Kinase Assay	IC50 values are evaluated using the PI3K (human) HTRF Assay. p85α/p110δ is obtained from Invitrogen. All other isoforms are produced in-house by co-expressing full-length human p85α with the indicated human full-length catalytic subunit containing a histidine tag at the N-terminus to allow purification. The PI3Ks are titrated and used at a concentration between their EC65-EC80 values. PI3K activity in immunoprecipitates is assayed using an antibody to the N-SH2 (N-Src homology 2) domain of p85α. Assays for other lipid kinases and protein kinases are performed by the National Centre for Protein Kinase Profiling and Invitrogen Drug Discovery Services[1].

Chemical Properties

Molecular Weight	393.53
Formula	C₁₇H₂₃N₅O₂S₂
Cas No.	1166227-08-2
Smiles	Cc1nc(NC(=O)N2CCC[C@H]2C(N)=O)sc1-c1csc(n1)C(C)(C)C
Relative Density.	1.354 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 1 mg/mL (2.54 mM), Sonication is recommended.

DMSO: 60 mg/mL (152.47 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.08 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5411 mL	12.7055 mL	25.4110 mL	127.0551 mL

DMSO

	1mg	5mg	10mg	50mg
5 mM	0.5082 mL	2.5411 mL	5.0822 mL	25.4110 mL
10 mM	0.2541 mL	1.2706 mL	2.5411 mL	12.7055 mL
20 mM	0.1271 mL	0.6353 mL	1.2706 mL	6.3528 mL
50 mM	0.0508 mL	0.2541 mL	0.5082 mL	2.5411 mL
100 mM	0.0254 mL	0.1271 mL	0.2541 mL	1.2706 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc