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Tyrphostin 23

Catalog No. T2063 CAS 118409-57-7

Synonyms: RG-50810, TX 825, Tyrphostin A23, AG18

Tyrphostin 23 (AG18) inhibits EGFR with IC50 of 35 μM.

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Tyrphostin 23, CAS 118409-57-7

Pack Size	Availability	Price/USD
10 mg	In stock	$ 54.00
25 mg	In stock	$ 124.00
50 mg	In stock	$ 236.00
100 mg	In stock	$ 355.00
1 mL * 10 mM (in DMSO)	In stock	$ 36.00

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Purity: 99.7%

Purity: 99.19%

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Description	Tyrphostin 23 (AG18) inhibits EGFR with IC50 of 35 μM.
Targets&IC50	EGFR:35 μM
In vivo	AG18 inhibits the volume-sensitive release of [3H] taurine in primary astrocyte cultures in a dose-dependent manner. Additionally, AG18 suppresses EGF-induced autophosphorylation of EGFR in A431 cells with an IC50 value of 15 μM and inhibits the proliferation of GH3 cells induced by EGF at a concentration of 10 μM. At the same concentration, AG18 also prevents the increase in ERK1/2 phosphorylation stimulated by Ghrelin in GH3 cells and counteracts 2xKCl-induced contractions, exerting maximal inhibitory effects. At 100 μM, AG18 blocks the activity of IKK stimulated by TNF-alpha and TPA in A549 epithelial cells. Furthermore, at a concentration of 300 μM, it dose-dependently inhibits the stimulation of ICAM-1 expression induced by TPA in A549 epithelial cells. Additionally, at this concentration, AG18 also hampers the TPA-stimulated NF-kappaB DNA-binding and ICAM-1 promoter activity in A549 epithelial cells.
Kinase Assay	EGF-Receptor Autophosphorylation: WGA-purified EGF receptor from A431 cells (0.5 μg/assay) is activated with EGF (800 nM) for 20 min at 4 ℃. The reaction is initiated by the addition of Mg(Ac)2 (60 mM), Tris-Mes buffer, pH 7.6 (50 mM), and [32P]ATP (20 pM, 5 μCi/assay). The reaction is conducted at either 4 ℃ or 15 ℃ and terminated by addition of sodium dodecyl sulfate (SDS) sample buffer (10% glycerol, 50 mM Tris, pH 6.8, 5% β-mercaptoethanol, and 3% SDS). The samples are run on a 8% SDS polyacrylamide gel (SDS-PAGE) (prepared from 30% acrylamide and 0.8% bis-(acrylamide) and contained 0.375 M Tris, pH 8.8, 0.1% SDS, 0.05% TEMED, and 0.46% ammonium persulfate). The gel is dried and autoradiography is perfromed with Agfa Curix RP2 X-ray film. The relevant radioactive bands are cut and counted in the Cerenkov mode. The fast phase of autophosphorylation continued for another 10 min. The extent of phosphorylation completed in the first 10 s at 15 ℃ comprises 1/3 of the total autophosphorylation signal and probably reflects the phosphorylation of the first site on the receptor. The 10-s interval is therefore chosen for use in subsequent autophosphorylation experiments.
Cell Research	GH3 cells are plated at 5 × 104 cells/well in media containing 2% charcoal-stripped FCS and various concentrations of ghrelin, desoctanoylated ghrelin and PMA or EGF for 72 hours with the addition of 2 μCi/well [3H]thymidine for a further 6 hours. A time-course of 24 hours, 48 hours and 72 hours is performed for ghrelin stimulation and 72 hours is selected for further experiments. Studies are also performed to investigate the effect of rat ghrelin or desoctanoyl ghrelin-induced proliferation and the effect of U0126, GF109203X, AG 18, wortmannin and H-89 upon ghrelin-induced MAPK stimulation. AG 18 at 10 μM is added 30 min before each treatment. Cells are harvested before counting in the presence of scintillation fluid using a Microbeta 1450 bcounter. Experiments are repeated at least three times.(Only for Reference)

Synonyms	RG-50810, TX 825, Tyrphostin A23, AG18
Molecular Weight	186.17
Formula	C10H6N2O2
CAS No.	118409-57-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 35 mg/mL (188 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 35 mg/mL (188 mM)

References and Literature

1. Gazit A, et al. J Med Chem, 1989, 32(10), 2344-2352. 2. Nanzer AM, et al. Eur J Endocrinol, 2004, 151(2), 233-240. 3. Mongin AA, et al. Am J Physiol, 1999, 276(5 Pt 1), C1226-1230. 4. Chen C, et al. Cell Signal, 2001, 13(8), 543-553.

Related compound libraries

This product is contained In the following compound libraries：

Tyrosine Kinase Inhibitor Library Membrane Protein-targeted Compound Library Anti-Cancer Active Compound Library Inhibitor Library Kinase Inhibitor Library Preclinical Compound Library Cytokine Inhibitor Library Bioactive Compounds Library Max Reprogramming Compound Library Bioactive Compound Library

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Keywords

Tyrphostin 23 118409-57-7 Angiogenesis JAK/STAT signaling Tyrosine Kinase/Adaptors EGFR inhibit RG-50810 Inhibitor HER1 Tyrphostin23 RG 50810 TX825 Epidermal growth factor receptor AG-18 AG 18 RG50810 TX 825 Tyrphostin A23 Tyrphostin A 23 AG18 TX-825 Tyrphostin A-23 ErbB-1 Tyrphostin-23 inhibitor

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