Shopping Cart

Your shopping cart is currently empty

Spermine

Name: Spermine
Brand: TargetMol
SKU: T3007
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: NSC 268508, Neuridine, Musculamine, Gerontine, 4,9-Diaza-1,12-dodecanediamine) Copy Product Info

🥰Excellent

Synonyms: NSC 268508, Neuridine, Musculamine, Gerontine, 4,9-Diaza-1,12-dodecanediamine

Catalog No. T3007 Copy Product Info

Purity: 99.96%

🥰Excellent

Spermine (Neuridine) is an endogenous polyamine in the human body. As a direct free radical scavenger, it exhibits antiviral activity and protects DNA from free radical damage; it is also a key regulator of cell proliferation, differentiation, and apoptosis. Spermine inhibits certain bacteria, particularly strains of Staphylococcus aureus. Spermine induces striatal neurotoxicity in a dose-dependent manner. Spermine reversibly inhibits DNA synthesis, mixed lymphocyte reactions, and the induction of cytolytic lymphocyte reactions in primary cultures of mouse splenocytes.

Cas No. 71-44-3

Pack Size	Price	USA Stock	Global Stock
50 mg	$30	In Stock	In Stock
100 mg	$40	-	In Stock
500 mg	$64	-	In Stock
1 g	$84	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:99.96%

Color:White

Resource Download

COA HPLC HNMR

Product Introduction

Spermine AI Summary

Spermine demonstrates multi-faceted bioactivity across various biological assays and targets. It exhibits potent inhibitory effects on polyamine transport in human breast cancer cells, particularly with a Ki value of 650.0 nM for putrescine, 920.0 nM for spermidine, and 2700.0 nM for spermine. Additionally, it effectively inhibits spermine transport in ZR-75-1 human breast cancer cells with a Ki of 200.0 nM, indicating its potential to disrupt polyamine metabolism in these cancer cells. In DNA binding studies, Spermine inhibits the fluorescence of ethidium bound to CT-DNA with a CC50 of 6800.0 nM. It also reduces the binding of [125I]PhTX-343-lysine to glutamate receptors in rat cortical membranes by 14.4%, and affects oxidative deamination by bovine serum amine oxidase (IC50 of 390.0 nM). Furthermore, it impacts ribozyme cleavage rates under specific conditions involving magnesium ions, showcasing its influence on RNA interactions. Toxicity assays reveal that Spermine has an in vitro cytotoxicity with an IC50 greater than 100,000.0 nM on F98 glioma cells. It binds to calf thymus DNA with an IC50 of 3,000.0 nM and demonstrates biodistribution in various tissues in mice with subcutaneous B16 melanomas. Additionally, it shows binding affinity to the mouse polyamine transporter on L1210 cells at 1340.0 nM and inhibits [3H]spermidine transport into MDA-MB-231 cells with a Ki of 280.0 nM. The compound’s extensive range of pKa values indicates multiple ionizable groups, influencing its bioactivity by affecting solubility, membrane permeability, and protein binding. It also modulates polyamine metabolism in various cell lines under different treatment conditions, suggesting a role in polyamine homeostasis. Bioactivity assays further highlight its inhibition of dexamethasone-induced excitotoxicity in NR1-1a/NR2A receptors, multiple carbonic anhydrase isoforms, monoamine oxidase enzymes, and NMDA receptor modulation. It also inhibits tubulin polymerization, showing varied effects based on the presence of MAPS. Spermine exhibits antibacterial activity with high MIC values, indicating moderate efficacy. It also enhances procaspase 2 activation and shows cytotoxicity against various cell lines, including CHO cells. Additionally, it demonstrates antiviral activity against HCV and SARS-CoV-2 with specific inhibition assays. Overall, Spermine presents a versatile compound with significant inhibitory and modulatory activities across a broad spectrum of biological pathways and targets, suggesting potential therapeutic applications in cancer, infectious diseases, and neuroprotection..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Spermine (Neuridine) is an endogenous polyamine in the human body. As a direct free radical scavenger, it exhibits antiviral activity and protects DNA from free radical damage; it is also a key regulator of cell proliferation, differentiation, and apoptosis. Spermine inhibits certain bacteria, particularly strains of Staphylococcus aureus. Spermine induces striatal neurotoxicity in a dose-dependent manner. Spermine reversibly inhibits DNA synthesis, mixed lymphocyte reactions, and the induction of cytolytic lymphocyte reactions in primary cultures of mouse splenocytes.
Targets & IC50	PRMT3:42.36 μM, PRMT6:3.81 μM, PRMT4:16.63 μM, PRMT8:9.22 μM, PRMT1:13.72 μM
In vitro	Methods: Mouse renal tubular epithelial cell lines were stimulated with TGF-β1 (10 ng/mL) for 24 hours to induce a fibrotic phenotype. Spermine (10 μM) was added 12 hours prior to TGF-β1 stimulation. Transcriptome sequencing was performed to assess gene expression changes. Results: Spermine reversed TGF-β1-induced expression of genes associated with ECM receptors and focal adhesion pathways. [1]
In vivo	Methods: A mouse model of renal fibrosis was established using unilateral renal ischemia-reperfusion injury. Spermine (1 mg/kg/day) was administered via intraperitoneal injection starting 2 days prior to surgery, with continuous dosing for 10 days (therapeutic experiments commenced on postoperative day 3). Results: Exogenous spermidine supplementation significantly attenuated renal fibrosis and downregulated fibrosis markers including fibronectin, collagen I, and α-SMA. [1]
Synonyms	NSC 268508, Neuridine, Musculamine, Gerontine, 4,9-Diaza-1,12-dodecanediamine

Chemical Properties

Molecular Weight	202.34
Formula	C₁₀H₂₆N₄
Cas No.	71-44-3
Smiles	NCCCNCCCCNCCCN
Relative Density.	0.925 g/cm3

Storage & Solubility Information

Storage

Keep away from direct sunlight,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: Slightly soluble

H2O: 257.5 mg/mL (1272.61 mM), Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	4.9422 mL	24.7109 mL	49.4218 mL	247.1088 mL
5 mM	0.9884 mL	4.9422 mL	9.8844 mL	49.4218 mL
10 mM	0.4942 mL	2.4711 mL	4.9422 mL	24.7109 mL
20 mM	0.2471 mL	1.2355 mL	2.4711 mL	12.3554 mL
50 mM	0.0988 mL	0.4942 mL	0.9884 mL	4.9422 mL
100 mM	0.0494 mL	0.2471 mL	0.4942 mL	2.4711 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.