Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Rotenone (Rotocide) is a natural product and a botanical insecticide. Rotenone is a mitochondrial electron transport complex I inhibitor that promotes the production of reactive oxygen species in mitochondria and induces apoptosis.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
500 mg | In stock | $ 42.00 | |
1 g | In stock | $ 50.00 | |
5 g | In stock | $ 89.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 46.00 |
Description | Rotenone (Rotocide) is a natural product and a botanical insecticide. Rotenone is a mitochondrial electron transport complex I inhibitor that promotes the production of reactive oxygen species in mitochondria and induces apoptosis. |
In vitro |
METHODS: Human leukemia cells HL-60 were treated with Rotenone (0-1000 nM) for 30 min and oxygen consumption was measured using a Clark oxygen electrode. RESULTS: Rotenone dose-dependently inhibited HL-60 cell respiration. Rotenone inhibited cellular respiration by more than 96% at 500 nM. [1] METHODS: 10th DIV cells were treated with Rotenone (20 nM) for 48 h. LDH levels were measured by LDH release assay. RESULTS: LDH release can be used as an indicator of general cytotoxicity. rotenone induced an increase in LDH release, and LDH activity increased more than 6-fold compared to the control group. LDH activity increased more than 6-fold compared to the control group.[2] |
In vivo |
METHODS: To establish a reproducible mouse model of Rotenone-induced Parkinson's disease (PD), Rotenone (2.5 mg/kg) was administered intraperitoneally to C57Bl/6 mice once daily for two weeks. RESULTS: Systemic exposure of mice to Rotenone resulted in progressive accumulation and regional spread of p129 aggregates, which preceded the maximal loss of DAn. [3] METHODS: To study neurotoxicity, Rotenone (30-100 mg/kg) was administered orally to C57BL/6 mice once daily for fifty-six days. RESULTS: The survival of Rotenone-treated mice at 30 mg/kg was unchanged from 28-56 days, although the survival of Rotenone-treated mice was reduced to approximately 70% within one week. 100 mg/kg Rotenone-treated mice showed a sudden decrease in survival after 28 days, and ultimately to approximately 15% after 56 days. A regimen of Rotenone given chronically at 30 mg/kg for 56 days is more useful for understanding the mechanisms of DA neurodegeneration. [4] |
Source |
Synonyms | Rotenon, Paraderil, Barbasco, Rotocide, Dactinol |
Molecular Weight | 394.42 |
Formula | C23H22O6 |
CAS No. | 83-79-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 39.4 mg/mL(100 mM)
You can also refer to dose conversion for different animals. More
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Rotenone 83-79-4 Apoptosis Autophagy Metabolism Dehydrogenase Mitochondrial Metabolism p53 Rotenon Paraderil Inhibitor Barbasco Rotocide inhibit Dactinol inhibitor