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Rotenone

Catalog No. T2970   CAS 83-79-4
Synonyms: Rotenon, Paraderil, Barbasco, Rotocide, Dactinol

Rotenone (Rotocide) is a natural product and a botanical insecticide. Rotenone is a mitochondrial electron transport complex I inhibitor that promotes the production of reactive oxygen species in mitochondria and induces apoptosis.

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Rotenone Chemical Structure
Rotenone, CAS 83-79-4
Pack Size Availability Price/USD Quantity
500 mg In stock $ 42.00
1 g In stock $ 50.00
5 g In stock $ 89.00
1 mL * 10 mM (in DMSO) In stock $ 46.00
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Purity: 99.48%
Purity: 99.39%
Purity: 98.49%
Purity: 97.48%
Purity: 96.99%
Purity: 91.16%
Purity: 89.65%
Purity: 89.58%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Rotenone (Rotocide) is a natural product and a botanical insecticide. Rotenone is a mitochondrial electron transport complex I inhibitor that promotes the production of reactive oxygen species in mitochondria and induces apoptosis.
In vitro METHODS: Human leukemia cells HL-60 were treated with Rotenone (0-1000 nM) for 30 min and oxygen consumption was measured using a Clark oxygen electrode.
RESULTS: Rotenone dose-dependently inhibited HL-60 cell respiration. Rotenone inhibited cellular respiration by more than 96% at 500 nM. [1]
METHODS: 10th DIV cells were treated with Rotenone (20 nM) for 48 h. LDH levels were measured by LDH release assay.
RESULTS: LDH release can be used as an indicator of general cytotoxicity. rotenone induced an increase in LDH release, and LDH activity increased more than 6-fold compared to the control group. LDH activity increased more than 6-fold compared to the control group.[2]
In vivo METHODS: To establish a reproducible mouse model of Rotenone-induced Parkinson's disease (PD), Rotenone (2.5 mg/kg) was administered intraperitoneally to C57Bl/6 mice once daily for two weeks.
RESULTS: Systemic exposure of mice to Rotenone resulted in progressive accumulation and regional spread of p129 aggregates, which preceded the maximal loss of DAn. [3]
METHODS: To study neurotoxicity, Rotenone (30-100 mg/kg) was administered orally to C57BL/6 mice once daily for fifty-six days.
RESULTS: The survival of Rotenone-treated mice at 30 mg/kg was unchanged from 28-56 days, although the survival of Rotenone-treated mice was reduced to approximately 70% within one week. 100 mg/kg Rotenone-treated mice showed a sudden decrease in survival after 28 days, and ultimately to approximately 15% after 56 days. A regimen of Rotenone given chronically at 30 mg/kg for 56 days is more useful for understanding the mechanisms of DA neurodegeneration. [4]
Source
Synonyms Rotenon, Paraderil, Barbasco, Rotocide, Dactinol
Molecular Weight 394.42
Formula C23H22O6
CAS No. 83-79-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 39.4 mg/mL(100 mM)

TargetMolReferences and Literature

1. Li N, et al. Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production. J Biol Chem. 2003 Mar 7;278(10):8516-25. 2. Radad K, et al. Rotenone induces cell death in primary dopaminergic culture by increasing ROS production and inhibiting mitochondrial respiration. Neurochem Int. 2006 Sep;49(4):379-86. 3. Rocha SM, et al. Rotenone induces regionally distinct α-synuclein protein aggregation and activation of glia prior to loss of dopaminergic neurons in C57Bl/6 mice. Neurobiol Dis. 2022 Jun 1;167:105685. 4. Inden M, et al. Parkinsonian rotenone mouse model: reevaluation of long-term administration of rotenone in C57BL/6 mice. Biol Pharm Bull. 2011;34(1):92-6. 5. Li Y, Sun H, Tian Z, et al. The determination of Plutella xylostella (L.) GSTs (PxGSTs) involved in the detoxification metabolism of Tolfenpyrad[J]. Pest Management Science. 2020.

TargetMolCitations

1. Li Y, Sun H, Tian Z, et al. The determination of Plutella xylostella (L.) GSTs (PxGSTs) involved in the detoxification metabolism of Tolfenpyrad. Pest Management Science. 2020 2. Yi Y, Gao K, Lin P, et al. Staphylococcus aureus-Induced Necroptosis Promotes Mitochondrial Damage in Goat Endometrial Epithelial Cells. Animals. 2022, 12(17): 2218. 3. Zhang R, Fang J, Qi T, et al.Maternal aging increases offspring adult body size via transmission of donut-shaped mitochondria.Cell Research.2023: 1-14. 4. Ma W, Sun Y, Yan R, et al.OXCT1 functions as a succinyltransferase, contributing to hepatocellular carcinoma via succinylating LACTB.Molecular Cell.2023

Related compound libraries

This product is contained In the following compound libraries:
Antiparasitic Natural Product Library Tobacco Monomer Library Anti-Neurodegenerative Disease Compound Library Toxic Compound Library FDA-Approved & Pharmacopeia Drug Library Anti-Aging Compound Library Anti-Colorectal Cancer Compound Library Ubiquitination Compound Library Natural Product Library Anti-Pancreatic Cancer Compound Library

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Keywords

Rotenone 83-79-4 Apoptosis Autophagy Metabolism Dehydrogenase Mitochondrial Metabolism p53 Rotenon Paraderil Inhibitor Barbasco Rotocide inhibit Dactinol inhibitor

 

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