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Ro5-3335

Catalog No. T4687 CAS 30195-30-3

Synonyms: CBFβ-Runx1 inhibitor II, Ro 5-3335

Ro 5-3335 is core binding factor (CBF) inhibitor; preferentially kills human leukemia cell lines with CBF fusion proteins (IC50 = 1.1 μM). It represses RUNX1/CBFβ-dependent transactivation in reporter assays and inhibits transcriptional regulation by RUNX1 and CBFβ. It also reduces the leukemia burden in a mouse model. Attenuates RUNX1-dependent hematopoiesis in zebrafish embryos. It also is a Tat antagonist and inhibits HIV-1 replication in vitro.

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Ro5-3335, CAS 30195-30-3

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

Targets&IC50

CBF:1.1 μM

Synonyms	CBFβ-Runx1 inhibitor II, Ro 5-3335
Molecular Weight	259.69
Formula	C13H10ClN3O
CAS No.	30195-30-3

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 100 mM

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Cunningham L, et al.Identification of benzodiazepine Ro5-3335 as an inhibitor of CBF leukemia through quantitative high throughput screen against RUNX1-CBFβ interaction.Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14592-7. 2. Cupelli LA, Hsu MC.The human immunodeficiency virus type 1 Tat antagonist, Ro 5-3335, predominantly inhibits transcription initiation from the viral promoter.J Virol. 1995 Apr;69(4):2640-3. 3. Okuda T,RUNX1/AML1: a central player in hematopoiesis.Int J Hematol. 2001 Oct;74(3):252-7. 4. Wang Y, Wang Q, Wang B, et al. Inhibition of EZH2 ameliorates bacteria-induced liver injury by repressing RUNX1 in dendritic cells[J]. Cell death & disease. 2020, 11(11): 1-13.

Citations

1. Lin W, Wan X, Sun A, et al. RUNX1/EGFR Pathway Contributes to STAT3 Activation and Tumor Growth Caused by Hyperactivated mTORC1. Molecular Therapy-Oncolytics. 2021 2. Wang Y, Wang Q, Wang B, et al. Inhibition of EZH2 ameliorates bacteria-induced liver injury by repressing RUNX1 in dendritic cells. Cell death & disease. 2020, 11(11): 1-13.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Ro5-3335 30195-30-3 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis Ro53335 CBFβ inhibit binding Ro-5-3335 CBFβ-Runx1 inhibitor II core Ro 5-3335 RUNX1 RUNX1-CBFB factor CBF interaction leukemia Inhibitor Ro5 3335 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.