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PD173955

Catalog No. T3063   CAS 260415-63-2
Synonyms: PD-173955, PD 173955

PD173955 is src family-selective tyrosine kinase inhibitor with IC50 of ~22 nM for Src, Yes and Abl kinase; less potent for FGFRα and no activity on InsR and PKC.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
PD173955 Chemical Structure
PD173955, CAS 260415-63-2
Pack Size Availability Price/USD Quantity
1 mg In stock $ 62.00
2 mg In stock $ 88.00
5 mg In stock $ 155.00
10 mg In stock $ 277.00
25 mg In stock $ 469.00
50 mg In stock $ 675.00
100 mg In stock $ 942.00
1 mL * 10 mM (in DMSO) In stock $ 166.00
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Purity: 99.28%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description PD173955 is src family-selective tyrosine kinase inhibitor with IC50 of ~22 nM for Src, Yes and Abl kinase; less potent for FGFRα and no activity on InsR and PKC.
Targets&IC50 Src:22 nM
Kinase Assay In Vitro Bcr-Abl Kinase Assays: Bcr-Abl complexed to SHIP2 is immunoprecipitated from cell lysates of K562 cells maintained in log-phase culture conditions. Complexes are collected on protein A-Sepharose, and complexes are washed three times in lysis buffer and then washed twice in abl kinase buffer [50 mM Tris (pH 8.0), 10 mM MgCl2, 1 mM DTT, 2 mM p-nitrophenylphosphate, and 2 μM ATP; New England Biolabs Buffer and protocol]. Kinase assays are performed with 10 μM [γ-32P]ATP/sample for 15–60 min at 30°C in the presence or absence of the indicated concentrations of drug. The reaction is stopped by the addition of SDS-PAGE sample buffer and heated at 100°C for 10 min. Proteins are separated on 7.5% SDS-polyacrylamide gels, gels are dried under vacuum, and phosphorylation is visualized by autoradiography on X-ray film.
Cell Research Cell growth is determined by two methods. For the [3H]thymidine assay, cells (104 cells/well) are cultured in 96-well, round-bottomed plates with diluted DMSO (control) or with varying concentrations of a specific compound that is resuspended in DMSO for 48 h at 37°C. [3H]Thymidine is added at a concentration of 1 μCi/well, and cells are incubated for an additional 18 h. Cells were harvested with the Unifilter system, scintillation fluid (25 μl/well) is added to each well, and [3H]thymidine incorporation is determined on a Packard Scintillation Counter. Data points for all assays are obtained in triplicate, and background incorporation from cell-free wells is determined and subtracted from all data points. For cell viability, control and drug-treated harvested cells are counted on a hemocytometer using the trypan blue dye exclusion method.(Only for Reference)
Synonyms PD-173955, PD 173955
Molecular Weight 443.35
Formula C21H16Cl2N4OS
CAS No. 260415-63-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: <1 mg/mL

DMSO: 12 mg/mL (27.1 mM)

TargetMolReferences and Literature

1. Wisniewski D, et al. Cancer Res. 2002, 62(15), 4244-4255. 2. Moasser MM, et al. Cancer Res. 1999, 59(24), 6145-6152.

Related compound libraries

This product is contained In the following compound libraries:
Tyrosine Kinase Inhibitor Library Anti-Cancer Active Compound Library Cytoskeletal Signaling Pathway Compound Library Anti-Parkinson's Disease Compound Library HIF-1 Signaling Pathway Compound Library Apoptosis Compound Library Bioactive Compounds Library Max Anti-Cancer Metabolism Compound Library Anti-Cancer Compound Library Reprogramming Compound Library

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Keywords

PD173955 260415-63-2 Angiogenesis Apoptosis Cytoskeletal Signaling Tyrosine Kinase/Adaptors Bcr-Abl Src inhibit Inhibitor PD-173955 PD 173955 inhibitor

 

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