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PAC-1

Catalog No. T2503   CAS 315183-21-2
Synonyms: Procaspase activating compound 1

PAC-1 (Procaspase activating compound 1) has been used in trials studying the treatment of Lymphoma, Melanoma, Solid Tumors, Breast Cancer, and Thoracic Cancers, among others.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
PAC-1 Chemical Structure
PAC-1, CAS 315183-21-2
Pack Size Availability Price/USD Quantity
10 mg In stock $ 43.00
25 mg In stock $ 75.00
50 mg In stock $ 125.00
100 mg In stock $ 226.00
500 mg In stock $ 913.00
1 mL * 10 mM (in DMSO) In stock $ 30.00
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Purity: 99.77%
Purity: 98.64%
Purity: 98%
Purity: 96.78%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description PAC-1 (Procaspase activating compound 1) has been used in trials studying the treatment of Lymphoma, Melanoma, Solid Tumors, Breast Cancer, and Thoracic Cancers, among others.
Targets&IC50 Procaspase-3:0.22 μM(EC50)
Kinase Assay In vitro procaspase-3 activation: Procaspase-3 is expressed and purified in Escherichia coli. Various concentrations of PAC-1 are added to 90 μL of a 50 ng/mL of procaspase-3 in caspase assay buffer in a 96-well plate, The plate is incubated for 12 hours at 37 °C. A 10 μL volume of a 2 mM solution of caspase-3 peptidic substrate acetyl Asp-Glu-Val-Asp-p-nitroanilide (Ac-DEVD-pNa) in caspase assay buffer is then added to each well. The plate is read every 2 minutes at 405 nm for 2 hours in a Spectra Max Plus 384 well plate reader. The slope of the linear portion for each well is determined, and the relative increase in activation from untreated control wells is calculated.
Cell Research Cells are exposed to various concentrations of PAC-1 for 72 hours. Cell death is quantified by the addition of MTS/PMS CellTiter 96 Cell Proliferation Assay reagent. The plates are incubated at 37 °C for approximately 1 hour (until the colored product formed), and the absorbance is measured at 490 n(Only for Reference)
Synonyms Procaspase activating compound 1
Molecular Weight 392.49
Formula C23H28N4O2
CAS No. 315183-21-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

1eq. HCl: 29.4 mg/mL (75 mM)

DMSO: 39.3 mg/mL (100 mM)

TargetMolReferences and Literature

1. Putt KS, et al. Nat Chem Biol, 2006, 2(10), 543-550. 2. Peterson QP, et al. J Mol Biol, 2009, 388(1), 144-158. 3. Seervi M, et al. Cell Death Dis, 2011, 2, e207.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Active Compound Library Anti-Cancer Clinical Compound Library Anti-Cancer Drug Library Bioactive Compounds Library Max Bioactive Lipid Compound Library Hematonosis Compound Library Anti-Cancer Compound Library Autophagy Compound Library Pyroptosis Compound Library Apoptosis Compound Library

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Keywords

PAC-1 315183-21-2 Apoptosis Autophagy Proteases/Proteasome Caspase Inhibitor Procaspase activating compound-1 PAC1 inhibit Procaspase activating compound 1 PAC 1 Procaspase activating compound1 inhibitor

 

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