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ON123300

Catalog No. T6920 CAS 1357470-29-1

ON123300 is a potent and multi-targeted kinase inhibitor for CDK4/Ark5/PDGFRβ/FGFR1/RET/Fyn (IC50: 3.9/5/26/26/9.2/11 nM).

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ON123300, CAS 1357470-29-1

Pack Size	Availability	Price/USD
1 mg	In stock	$ 48.00
2 mg	In stock	$ 70.00
5 mg	In stock	$ 102.00
10 mg	In stock	$ 177.00
25 mg	In stock	$ 358.00
50 mg	In stock	$ 535.00
100 mg	In stock	$ 773.00
500 mg	In stock	$ 1,580.00
1 mL * 10 mM (in DMSO)	In stock	$ 102.00

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Purity: 99.53%

Purity: 99.22%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	ON123300 is a potent and multi-targeted kinase inhibitor for CDK4/Ark5/PDGFRβ/FGFR1/RET/Fyn (IC50: 3.9/5/26/26/9.2/11 nM).
Targets&IC50	Ark5:5nM, PDGFRβ:26nM, Fyn:11 nM, CDK4:3.9nM, FGFR1:26nM, RET:9.2nM
Kinase Assay	CDK1 kinase activity is tested by the CDK1/cyclin B complex purified from baculovirus to phosphorylate a biotinylated peptide substrate containing the consensus phosphorylation site for histone H1, which is phosphorylated in vivo by CDK1. Inhibition of CDK1 activity is measured by observing a decreased amount of 33P-γ-ATP incorporation into the immobilized substrate in streptavidin-coated 96-well scintillating microplates. CDK1 enzyme is diluted in 50 mM Tris-HCl (pH 8), 10 mM MgCl2, 0.1 mM Na3VO4, 1 mM DTT, 1% DMSO, 0.25 μM peptide, 0.1 μCi per well 33P-γ-ATP, and 5 μM ATP in the presence or absence of various concentrations of JNJ-7706621 and incubated at 30 °C for 1 hour. The reaction is terminated by washing with PBS containing 100 mM EDTA and plates are counted in a scintillation counter. IC50 is determined by Linear regression analysis of the percent inhibition by JNJ-7706621.

Molecular Weight	429.52
Formula	C24H27N7O
CAS No.	1357470-29-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 28 mg/mL (65.2 mM)

References and Literature

1. Divakar SK, et al .Leukemia. 2016 Jan;30(1):86-93. 2. Perumal D, et al. Cancer Res. 2016 Mar 1;76(5):1225-36. 3. Zhang X, et al. Mol Cancer Ther. 2014 May;13(5):1105-16.

Related compound libraries

This product is contained In the following compound libraries：

Tyrosine Kinase Inhibitor Library Anti-Cancer Clinical Compound Library Anti-Cancer Drug Library Inhibitor Library Anti-Cancer Active Compound Library Kinase Inhibitor Library Drug Repurposing Compound Library Bioactive Compounds Library Max Epigenetics Compound Library ReFRAME Related Library

Related Products

Related compounds with same targets

AZD4547 FGFR3-IN-7 ENMD-2076 KW-2449 FGFR4-IN-16 EOC317 PP58 SUN11602

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

ON123300 1357470-29-1 Angiogenesis Apoptosis Cell Cycle/Checkpoint Chromatin/Epigenetic JAK/STAT signaling PI3K/Akt/mTOR signaling Stem Cells Tyrosine Kinase/Adaptors FGFR c-RET JAK CDK Src PDGFR AMPK Platelet-derived growth factor receptor AMP-activated protein kinase FGFR1 kinase brain Ark5 U87 inhibit multi RET tumor Inhibitor glioma Cyclin dependent kinase CDK4 FYN RTK penetration PDGFRβ ON-123300 CDK6 ON 123300 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.