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Anti-FGFR1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00674
Alias N-sam, FLT-2, FGFR1 β (IIIc), FGFR-1, CD331, bFGF-R-1, BFGFR

Anti-FGFR1 Polyclonal Antibody is a Rabbit antibody targeting FGFR1. Anti-FGFR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-FGFR1 Polyclonal Antibody

Anti-FGFR1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00674Alias N-sam, FLT-2, FGFR1 β (IIIc), FGFR-1, CD331, bFGF-R-1, BFGFR
Anti-FGFR1 Polyclonal Antibody is a Rabbit antibody targeting FGFR1. Anti-FGFR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-FGFR1 Polyclonal Antibody is a Rabbit antibody targeting FGFR1. Anti-FGFR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasN-sam, FLT-2, FGFR1 β (IIIc), FGFR-1, CD331, bFGF-R-1, BFGFR
Ig Type
IgG
Reactivity
Human,Mouse,Rat,Cow (predicted:Chicken,Dog)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FGFR1) Polyclonal Antibody, Unconjugated (TMAB-00674) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
MCF-7 (Human) Cell Lysate at 30 μg
Primary: Anti-CD331 (TMAB-00674) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 130 kDa
3. Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FGFR1) Polyclonal Antibody, Unconjugated (TMAB-00674) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FGFR1) Polyclonal Antibody, Unconjugated (TMAB-00674) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human meningioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FGFR1) Polyclonal Antibody, Unconjugated (TMAB-00674) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundFibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human BFGFR
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Receptor Tyrosine Kinases,FGF,Neurogenesis,SARS Coronavirus,FGF Receptors,FGF
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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