store at low temperture,keep away from direct sunlight
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
Alantolactone(Alant camphor), a sesquiterpene lactone, has potential activity against triple-negative breast Y MDA-MB-231 cells by suppressing the signal transducer and activator of transcription 3 (STAT3) signaling pathway.
Description | Alantolactone(Alant camphor), a sesquiterpene lactone, has potential activity against triple-negative breast Y MDA-MB-231 cells by suppressing the signal transducer and activator of transcription 3 (STAT3) signaling pathway. |
In vitro | Alantolactone selectively suppresses STAT3 activation and exhibits potent anticancer activity in MDA-MB-231 cells. Alantolactone effectively suppressed both constitutive and inducible STAT3 activation at tyrosine 705. It decreased STAT3 translocation to the nucleus, its DNA-binding, and STAT3 target gene expression. Alantolactone significantly inhibits STAT3 activation with a marginal effect on MAPKs and on NF-κB transcription; however, this effect is not mediated by inhibiting STAT3 upstream kinases. SHP-1, SHP-2, and PTEN, which are protein tyrosine phosphatases (PTPs) were not affected by alantolactone. Alantolactone treatment resulted in the inhibition of migration, invasion, adhesion, and colony formation[1]. Alantolactone could induce activin signaling and activin target gene expression. Alantolactone activated the activin signaling pathway in a cancer cell line. It could promote SMAD2/3 nuclear translocation in a very short time[3]. |
In vivo | In vivo administration of alantolactone inhibited the growth of human breast xenograft tumors[1]. Alantolactone exerts no toxic effect on liver and kidneys in vivo, The drug was well tolerated by mice and no mortality or any sign of pharmacotoxicity was found at a dose of 100 mg/kg during all the experimental periods. Alantolactone can cross blood-brain barrier[2]. |
Cell Research | Cell viability was evaluated using MTT assay. The cells were seeded into 96-well plates and maintained at 37 °C for 24 h. The cells were treated with alantolactone for 24 h. The MTT solution (0.5 mg/mL) was added to each well, and the cells were incubated for another 3 h. The MTT formazan crystals were dissolved in DMSO. The results were obtained by measuring the absorbance at a wavelength of 540 nm using a microplate reader.(Only for Reference) |
Synonyms | Alant camphor, Helenine, Inula camphor, (+)-Alantolactone, helenin, Eupatal |
Molecular Weight | 232.33 |
Formula | C15H20O2 |
CAS No. | 546-43-0 |
store at low temperture,keep away from direct sunlight
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
DMSO: 23.2 mg/mL(100 mM)
Ethanol: 46 mg/mL (198 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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Alantolactone 546-43-0 Apoptosis JAK/STAT signaling Stem Cells STAT TGF-beta/Smad Alant camphor Helenine Transforming growth factor beta inhibit Inhibitor Inula camphor (+)-Alantolactone helenin Eupatal inhibitor