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Alantolactone

Catalog No. T2896 CAS 546-43-0

Synonyms: Alant camphor, Helenine, Inula camphor, (+)-Alantolactone, helenin, Eupatal

Alantolactone(Alant camphor), a sesquiterpene lactone, has potential activity against triple-negative breast Y MDA-MB-231 cells by suppressing the signal transducer and activator of transcription 3 (STAT3) signaling pathway.

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Alantolactone, CAS 546-43-0

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Alantolactone(Alant camphor), a sesquiterpene lactone, has potential activity against triple-negative breast Y MDA-MB-231 cells by suppressing the signal transducer and activator of transcription 3 (STAT3) signaling pathway.
In vitro	Alantolactone selectively suppresses STAT3 activation and exhibits potent anticancer activity in MDA-MB-231 cells. Alantolactone effectively suppressed both constitutive and inducible STAT3 activation at tyrosine 705. It decreased STAT3 translocation to the nucleus, its DNA-binding, and STAT3 target gene expression. Alantolactone significantly inhibits STAT3 activation with a marginal effect on MAPKs and on NF-κB transcription; however, this effect is not mediated by inhibiting STAT3 upstream kinases. SHP-1, SHP-2, and PTEN, which are protein tyrosine phosphatases (PTPs) were not affected by alantolactone. Alantolactone treatment resulted in the inhibition of migration, invasion, adhesion, and colony formation[1]. Alantolactone could induce activin signaling and activin target gene expression. Alantolactone activated the activin signaling pathway in a cancer cell line. It could promote SMAD2/3 nuclear translocation in a very short time[3].
In vivo	In vivo administration of alantolactone inhibited the growth of human breast xenograft tumors[1]. Alantolactone exerts no toxic effect on liver and kidneys in vivo, The drug was well tolerated by mice and no mortality or any sign of pharmacotoxicity was found at a dose of 100 mg/kg during all the experimental periods. Alantolactone can cross blood-brain barrier[2].
Cell Research	Cell viability was evaluated using MTT assay. The cells were seeded into 96-well plates and maintained at 37 °C for 24 h. The cells were treated with alantolactone for 24 h. The MTT solution (0.5 mg/mL) was added to each well, and the cells were incubated for another 3 h. The MTT formazan crystals were dissolved in DMSO. The results were obtained by measuring the absorbance at a wavelength of 540 nm using a microplate reader.(Only for Reference)

Synonyms	Alant camphor, Helenine, Inula camphor, (+)-Alantolactone, helenin, Eupatal
Molecular Weight	232.33
Formula	C15H20O2
CAS No.	546-43-0

Storage

store at low temperture,keep away from direct sunlight

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 23.2 mg/mL(100 mM)

Ethanol: 46 mg/mL (198 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Chun J, et al. Cancer Lett. 2015, 357(1):393-403. 2. Khan M, et al. IUBMB Life. 2012, 64(9):783-94. 3. Shi Y, et al. J Biomol Screen. 2011, 16(5):525-35.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Alantolactone 546-43-0 Apoptosis JAK/STAT signaling Stem Cells STAT TGF-beta/Smad Alant camphor Helenine Transforming growth factor beta inhibit Inhibitor Inula camphor (+)-Alantolactone helenin Eupatal inhibitor

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