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α-Cyano-4-hydroxycinnamic acid

Catalog No. T7460 CAS 28166-41-8

Synonyms: α-Cyano-4-hydroxycinnamic acid(α-CHCA), 2-氰基-4-羟基

α-Cyano-4-hydroxycinnamic acid (α-CHCA) is an inhibitor of monocarboxylate transporter 1 (MCT1) that blocks pyruvate transport in rat heart mitochondria (IC50 : 1.5 μM).

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α-Cyano-4-hydroxycinnamic acid, CAS 28166-41-8

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Purity: 98%

Biological Description

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Storage & Solubility Information

Description	α-Cyano-4-hydroxycinnamic acid (α-CHCA) is an inhibitor of monocarboxylate transporter 1 (MCT1) that blocks pyruvate transport in rat heart mitochondria (IC50 : 1.5 μM).
In vitro	The effectiveness of CHC in glioma cells appears to be dependent on Monocarboxylate transporters (MCTs) membrane expression.?MCT1 downregulation showed similar effects on different glioma cells, supporting CHC as an MCT1 inhibitor.?There was a synergistic effect when combining CHC with temozolomide treatment in U251 cells.?In the CAM in vivo model, CHC decreased the size of tumors and the number of blood vessels formed[1].
Cell Research	Cells were plated into 96-well plates, at a density of 3 × 10^3 cells per well.?The effect of treatment with CHC (0.6–12 mM) on cell number (total biomass) was determined at 24, 48, and 72 h by the?sulforhodamine B assay (SRB, TOX-6).?IC50 values (CHC concentration that corresponds to 50% of cell growth inhibition) were estimated from 3 independent experiments, each one in triplicate, using GraphPad Software.?Cell proliferation assay was performed as previously described32 and assessed under the treatment conditions previously described, for 5 mM and 10 mM of CHC.?After CHC treatment, cells were incubated with BrdU and its incorporation was assessed at 450 nm (λref = 655 nm) .?Cell growth (total biomass) and cell proliferation for glioma siMCT1 cells and the effect of CHC (1.25–15 mM) on cell number were performed[1].

Synonyms	α-Cyano-4-hydroxycinnamic acid(α-CHCA), 2-氰基-4-羟基
Molecular Weight	189.17
Formula	C10H7NO3
CAS No.	28166-41-8

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 37mg/mL(195.59mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Miranda-Goncalves V , Honavar M , Pinheiro C , et al. Monocarboxylate transporters (MCTs) in gliomas: expression and exploitation as therapeutic targets[J]. Neuro-Oncology, 2013, 15(2):172-188. 2. Colen C B , Shen Y , Ghoddoussi F , et al. Metabolic Targeting of Lactate Efflux by Malignant Glioma Inhibits Invasiveness and Induces Necrosis: An In Vivo Study[J]. Neoplasia, 2011, 13(7):620-632.

Citations

1. Tong Y, Guo C, Liu Z, et al. In situ localization of tris-(2, 3-dibromopropyl) isocyanurate in mouse organs by MALDI-IMS with auxiliary matrix strategy. Talanta. 2021: 122723.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Inhibitor Library Ion Channel Inhibitor Library Bioactive Compound Library

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Related compounds with same targets

α-Cyano-4-hydroxycinnamic acid MCT1-IN-3 AZD0095 VB124 7ACC1 BAY-8002 AZD3965 7ACC2

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Keywords

α-Cyano-4-hydroxycinnamic acid 28166-41-8 离子通道 Monocarboxylate transporter matrix-assisted MALDI-TOF matrix Inhibitor lactate applications MCTs pyruvate laser desorption spectrometry ionization Monocarboxylate Transporter transporter α Cyano 4 hydroxycinnamic acid peptide α-Cyano-4-hydroxycinnamic acid(α-CHCA) inhibit αCyano4hydroxycinnamic acid facilitate inhibitor

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