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Niflumic acid

Name: Niflumic acid
Brand: TargetMol
SKU: T0693
Price: 44 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0693Cas No. 4394-00-7

Alias Nifluril, Landruma, Forenol

Niflumic acid (Nifluril) is a Ca2+-activated Cl- channel blocker and an analgesic and anti-inflammatory agent used in the therapy of rheumatoid arthritis.

Niflumic acid

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Purity: 99.47%

Catalog No. T0693Alias Nifluril, Landruma, ForenolCas No. 4394-00-7

Niflumic acid (Nifluril) is a Ca2+-activated Cl- channel blocker and an analgesic and anti-inflammatory agent used in the therapy of rheumatoid arthritis.

Pack Size	Price	USA Warehouse	Global Warehouse
50 mg	$44	In Stock	In Stock
100 mg	$54	In Stock	In Stock
200 mg	$75	In Stock	In Stock
500 mg	$119	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$50	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.47%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Niflumic acid (Nifluril) is a Ca2+-activated Cl- channel blocker and an analgesic and anti-inflammatory agent used in the therapy of rheumatoid arthritis.
Targets&IC50	COX-2:100 nM
In vivo	Niflumic acid suppresses calcium-activated currents on the basolateral membrane of rat pancreatic exocrine cells with an IC50 of 50 μM. It dose-dependently and reversibly activates the calcium-activated potassium (KCa) channels. Niflumic acid induces a spontaneous transient concentration-dependent inhibition of inward currents (STIC, calcium-activated chloride currents) amplitude. It inhibits calcium-activated chloride channels with a Ki of 17 mM. Additionally, Niflumic acid suppresses ICl(Ca) under the permeabilizing effect of Ca2+ on oocytes through Ca2+ ionophore A23187, suggesting the inhibition of ICl(Ca) results from direct interaction with Cl- channels, not by disruption of Ca2+ entry through voltage-dependent Ca2+ channels. Niflumic acid attenuates norepinephrine and caffeine-induced inward currents (IO(Ca)) with an IC50 of 6.6 μM, which is less effective than that on spontaneous currents. It exhibits a voltage-dependent inhibition of the amplitude of spontaneous transient currents (STIC), with IC50 values of 1.1 μM at +50 mV and 2.3 μM at -50 mV. Furthermore, Niflumic acid reduces airway hyperresponsiveness and eosinophilic infiltration, as well as inhibits IL-13 induced goblet cell hyperplasia. Following the IL-13 challenge, Niflumic acid hampers the overexpression of the MUC5AC gene (a marker of goblet cell proliferation) and levels of chemokines in bronchoalveolar lavage fluid. It also represses the activation of JAK/STAT6, AK2, and the expression of chemokines in epithelial cells.
Synonyms	Nifluril, Landruma, Forenol

Chemical Properties

Molecular Weight	282.22
Formula	C₁₃H₉F₃N₂O₂
Cas No.	4394-00-7
Smiles	OC(=O)C1=CC=CN=C1NC1=CC=CC(=C1)C(F)(F)F
Relative Density.	1.3935 g/cm3 (Estimated)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (212.6 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.09 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.5433 mL	17.7167 mL	35.4333 mL	177.1667 mL
5 mM	0.7087 mL	3.5433 mL	7.0867 mL	35.4333 mL
10 mM	0.3543 mL	1.7717 mL	3.5433 mL	17.7167 mL
20 mM	0.1772 mL	0.8858 mL	1.7717 mL	8.8583 mL
50 mM	0.0709 mL	0.3543 mL	0.7087 mL	3.5433 mL
100 mM	0.0354 mL	0.1772 mL	0.3543 mL	1.7717 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc