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RWJ 50271

Name: RWJ 50271
Brand: TargetMol
SKU: T12783
Price: 129 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12783Cas No. 162112-37-0

RWJ 50271 is a selective lymphocyte function-associated antigen-1/intercellular adhesion molecule-1(LFA-1/ICAM-1) interaction inhibitor( IC50 of 5.0 μM in HL60 cells).

RWJ 50271

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Purity: 99.09%

Catalog No. T12783Cas No. 162112-37-0

RWJ 50271 is a selective lymphocyte function-associated antigen-1/intercellular adhesion molecule-1(LFA-1/ICAM-1) interaction inhibitor( IC50 of 5.0 μM in HL60 cells).

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$129	In Stock	In Stock
5 mg	$328	In Stock	In Stock
10 mg	$496	In Stock	In Stock
25 mg	$786	In Stock	In Stock
50 mg	$1,070	In Stock	In Stock
100 mg	$1,380	In Stock	In Stock
200 mg	$1,880	-	In Stock
1 mL x 10 mM (in DMSO)	$363	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.09%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

RWJ 50271 AI Summary

RWJ 50271 inhibits the adhesion of HL-60 cells to purified ICAM-1 by LFA-1 with an IC50 value of 5000.0 nM. It exhibits antiviral activity by inhibiting SARS-CoV-2 induced cytotoxicity of Caco-2 cells at a 10 µM concentration after 48 hours, achieving an inhibition rate of 14.94%. Additionally, it demonstrates a 21.5% inhibition of SARS-CoV-2 3CL-Pro protease at a 20 µM concentration through a Fluorescence Resonance Energy Transfer (FRET) assay with a peptide substrate. In VERO-6 cells, RWJ 50271 shows a 0.75% inhibition in preventing SARS-CoV-2 induced cytotoxicity after 48 hours of exposure. Furthermore, it exhibits 6.02% inhibition of the human HDAC6 enzyme using a commercial peptide substrate and -9.53% inhibition with a custom peptide substrate, indicating potential as an HDAC6 inhibitor..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	RWJ 50271 is a selective lymphocyte function-associated antigen-1/intercellular adhesion molecule-1(LFA-1/ICAM-1) interaction inhibitor( IC50 of 5.0 μM in HL60 cells).
Targets&IC50	LFA1-ICAM1 (HL60 cells):5.0 μM

Chemical Properties

Molecular Weight	410.41
Formula	C₁₈H₁₇F₃N₄O₂S
Cas No.	162112-37-0
Smiles	Cc1c(cnn1-c1nc(cs1)-c1cccc(c1)C(F)(F)F)C(=O)NCCCO
Relative Density.	1.44 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 230 mg/mL (560.42 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (12.18 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4366 mL	12.1829 mL	24.3659 mL	121.8294 mL
5 mM	0.4873 mL	2.4366 mL	4.8732 mL	24.3659 mL
10 mM	0.2437 mL	1.2183 mL	2.4366 mL	12.1829 mL
20 mM	0.1218 mL	0.6091 mL	1.2183 mL	6.0915 mL
50 mM	0.0487 mL	0.2437 mL	0.4873 mL	2.4366 mL
100 mM	0.0244 mL	0.1218 mL	0.2437 mL	1.2183 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc