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PF-6274484

Name: PF-6274484
Brand: TargetMol
SKU: T22396
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T22396Cas No. 1035638-91-5

Alias PF 6274484

PF-6274484, a high affinity, irreversible covalent inhibitor of EGFR kinase with Ki of 0.14 nM, inhibits the autophosphorylation of wild-type EGFR in A549 cells and EGFRL858R/T790M in H1975 cells with IC50 of 5.8 nM and 6.6 nM, respectively.

PF-6274484

🥰Excellent

Purity: 97.71%

Catalog No. T22396Alias PF 6274484Cas No. 1035638-91-5

Pack Size	Price	Availability
2 mg	$34	In Stock
5 mg	$55	In Stock
10 mg	$93	In Stock
25 mg	$197	In Stock
50 mg	$369	In Stock
100 mg	$549	In Stock
500 mg	$1,190	In Stock
1 mL x 10 mM (in DMSO)	$61	In Stock

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Batch Information

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Purity:97.71%

Resource Download

COA HPLC LCMS HNMR

Product Introduction

Bioactivity

Description	PF-6274484, a high affinity, irreversible covalent inhibitor of EGFR kinase with Ki of 0.14 nM, inhibits the autophosphorylation of wild-type EGFR in A549 cells and EGFRL858R/T790M in H1975 cells with IC50 of 5.8 nM and 6.6 nM, respectively.
Targets&IC50	EGFR (WT):0.18 nM (Ki), EGFR:0.14 nM(Ki)
Synonyms	PF 6274484

Chemical Properties

Molecular Weight	372.78
Formula	C₁₈H₁₄ClFN₄O₂
Cas No.	1035638-91-5
Smiles	COc1cc2ncnc(Nc3ccc(F)c(Cl)c3)c2cc1NC(=O)C=C
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 65 mg/mL (174.37 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6825 mL	13.4127 mL	26.8255 mL	134.1274 mL
5 mM	0.5365 mL	2.6825 mL	5.3651 mL	26.8255 mL
10 mM	0.2683 mL	1.3413 mL	2.6825 mL	13.4127 mL
20 mM	0.1341 mL	0.6706 mL	1.3413 mL	6.7064 mL
50 mM	0.0537 mL	0.2683 mL	0.5365 mL	2.6825 mL
100 mM	0.0268 mL	0.1341 mL	0.2683 mL	1.3413 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc