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Anti-EGFR Polyclonal Antibody 3
😃Good
Catalog No. TMAB-00597
Anti-EGFR Polyclonal Antibody 3 is a Rabbit antibody targeting EGFR. Anti-EGFR Polyclonal Antibody 3 can be used in WB,IHC-Fr,IF,ELISA,IHC-P.
Anti-EGFR Polyclonal Antibody 3
Anti-EGFR Polyclonal Antibody 3
😃Good
Catalog No. TMAB-00597
Anti-EGFR Polyclonal Antibody 3 is a Rabbit antibody targeting EGFR. Anti-EGFR Polyclonal Antibody 3 can be used in WB,IHC-Fr,IF,ELISA,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-Fr,IF,ELISA,IHC-P Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EGFR) Polyclonal Antibody, Unconjugated (TMAB-00597) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EGFR) Polyclonal Antibody, Unconjugated (TMAB-00597) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EGFR) Polyclonal Antibody, Unconjugated (TMAB-00597) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human MDA-MB-231 cell lysates Primary: Anti-EGFR (TMAB-00597) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 175 kDa Observed band size: 170 kDa 5. 25 μg total protein per Lane of various lysates probed with EGFR polyclonal antibody, unconjugated (TMAB-00597) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | WB,IHC-Fr,IF,ELISA,IHC-P |
| Recommended Dose | WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000; IHC-P: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Isoform 2: Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| Immunogen | KLH conjugated synthetic peptide: human EGFR |
| Antigen Species | Human |
| Gene ID | 1956 |
| Uniprot ID | |
| Biology Area | Receptor Tyrosine Kinases,EGF,egfr,SARS Coronavirus,Cell differentiation,Receptors,Receptor tyrosine kinases,Growth factor receptors,EGF |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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