Menaquinone-4 (Vitamin K2) is a vitamin K compound used as a hemostatic agent, and also as adjunctive therapy for the pain of osteoporosis.

Vitamin K supplementation reversed the high fat diet induced bone deterioration by modulating osteoblast and osteoclast activities and prevent bone loss in a high-fat diet-induced obese mice[1].

Forty-two male, 4-week-old C57BL/6J mice.?an automated constant-temperature humidity system was used to maintain a temperature of 23°C ± 2°C and humidity of 60% ± 3% for the animals.?The animals were provided with feed and drink ad libitum.?For environmental adaptation, the animals had 1 week of circulation, and then were provided with the experimental diet after being randomly divided into 6 groups (7 animals in each group;?randomized block design).?The AIN-93G diets consisted of a normal diet (N), normal diet + vitamin K1 (N-K1), normal diet + vitamin K2 (N-K2), 45% high-fat diet (HF), 45% high-fat diet + vitamin K1 (HF-K1), and a 45% high-fat diet + vitamin K2 (HF-K2).?The vitamin K1 and vitamin K2 contents were 200 mg/1,000 g, and the diet was provided in pellet form.?Food intake amount was measured every other day.?Body weight was measured once a week, and the food efficiency ratio (FER) was calculated by dividing the increased body weight from day1 to the final day by the food intake amount during the experiment period.?For fat amount measurement, the epididymal fat, perirenal fat, and retroperitoneal fat were extracted from dead animal subjects, were washed with 0.9% NaCl, dried by filter paper, and then were weighed[1].

DMSO: 10.33 mg/mL (23.23 mM), Sonication is recommended.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (2.25 mM), Sonication is recommended.