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Hyodeoxycholic acid

Name: Hyodeoxycholic acid
Brand: TargetMol
SKU: T2968
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2968Cas No. 83-49-8

Alias α-Hyodeoxycholic Acid, NSC 60672, HDCA

Hyodeoxycholic acid (NSC 60672) has been used in trials studying the treatment of Hypercholesterolemia.

Hyodeoxycholic acid

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Purity: 98.82%

Catalog No. T2968Alias α-Hyodeoxycholic Acid, NSC 60672, HDCACas No. 83-49-8

Hyodeoxycholic acid (NSC 60672) has been used in trials studying the treatment of Hypercholesterolemia.

Pack Size	Price	USA Warehouse	Global Warehouse
500 mg	$35	-	In Stock
1 g	$48	-	In Stock
5 g	$78	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.82%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Hyodeoxycholic acid AI Summary

Hyodeoxycholic acid exhibits diverse biological activities across multiple domains. It shows strong binding affinity (210,000 Kb M-1) and a sensitivity change (0.4) towards cyclodextrin-conjugated peptide (with pyrene) at 25°C. Notably, it inhibits glucuronosyltransferase activity in human colon cancer cell line HT-29 at 5 µM concentration with 75.2% activity. The compound is highly soluble in water (15,000 nM) and forms micelles at a Critical Micellar Concentration of 14.0 mM with pH stability varying at a pKa of 5.0 and a critical micellar pH of 8.1. It has high lipophilicity with a logP of 4.27 and a relative lipophilicity value of 0.89. In terms of receptor activity, Hyodeoxycholic acid acts as an agonist at human TGR5 in CHO cells (EC50 of 31,600 nM) with 79.7% efficacy relative to lithocholic acid. It exhibits cytotoxic effects in human HET-1A and HuH7 cells (500 µM) with 0.882% and 0.738% activity, respectively. The compound demonstrates varying activities against different isoforms of UDP-glucuronosyltransferases (UGTs), showing higher activity with UGT1A8 and UGT2B7. It is also a substrate for Oatp1 transport protein with a Km value of 17,500 nM in Oatp1-expressing HeLa cells. Hyodeoxycholic acid exhibits antiplasmodial activity against Plasmodium falciparum 3D7, antitrypanosomal activity against Trypanosoma brucei brucei, and cytotoxicity against human WI38 cells. It has agonistic and antagonistic activities based on VDR-LBD and VP16 tagged-VDR-LBD assays, with EC50 and IC50 values greater than 150,000 nM and 50,000 nM, respectively. Additionally, it demonstrates antibacterial activity against Clostridium difficile NAP1 with 500 µM required for inhibition, antiviral efficacy against SARS-CoV-2 by reducing cytotoxicity in VERO-6 and Caco-2 cells at 10 µM, and inhibiting the SARS-CoV-2 3CL-Pro protease by -1.852% at 20 µM. Lastly, it shows weak inhibitory activity on human HDAC6 with commercial and custom peptide substrates (-3.31% and -1.14%)..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Hyodeoxycholic acid (NSC 60672) has been used in trials studying the treatment of Hypercholesterolemia.
Targets&IC50	TGR5/GPCR19:31.6 µM(EC50)
Synonyms	α-Hyodeoxycholic Acid, NSC 60672, HDCA

Chemical Properties

Molecular Weight	392.57
Formula	C₂₄H₄₀O₄
Cas No.	83-49-8
Smiles	[H][C@@]12CC[C@H]([C@H](C)CCC(O)=O)[C@@]1(C)CC[C@@]1([H])[C@@]2([H])C[C@H](O)[C@]2([H])C[C@H](O)CC[C@]12C
Relative Density.	0.9985 g/cm3 (Estimated)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (127.37 mM), Sonication is recommended.

Ethanol: 72 mg/mL (183.41 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.09 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.5473 mL	12.7366 mL	25.4732 mL	127.3658 mL
5 mM	0.5095 mL	2.5473 mL	5.0946 mL	25.4732 mL
10 mM	0.2547 mL	1.2737 mL	2.5473 mL	12.7366 mL
20 mM	0.1274 mL	0.6368 mL	1.2737 mL	6.3683 mL
50 mM	0.0509 mL	0.2547 mL	0.5095 mL	2.5473 mL
100 mM	0.0255 mL	0.1274 mL	0.2547 mL	1.2737 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc