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hVEGF-IN-1

Catalog No. T4288 CAS 1637443-98-1

hVEGF-IN-1 inhibits human VEGF-A translation and has antitumor activity.

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hVEGF-IN-1, CAS 1637443-98-1

Pack Size	Availability	Price/USD
1 mg	In stock	$ 97.00
5 mg	In stock	$ 222.00
10 mg	In stock	$ 313.00
25 mg	In stock	$ 529.00
50 mg	In stock	$ 765.00
100 mg	In stock	$ 1,070.00
500 mg	In stock	$ 2,150.00
1 mL * 10 mM (in DMSO)	In stock	$ 285.00

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Purity: 98.85%

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Description	hVEGF-IN-1 inhibits human VEGF-A translation and has antitumor activity.
In vitro	hVEGF-IN-1 has a markedly selective interaction with the G-rich region within the 5′-UTR of hVEGF-A mRNA and destabilizes the G-quadruplex structure. hVEGF-IN-1 binds to the IRES-A (WT) (Kd: 0.928 μM) and binds to the hairpin DNA (Kd: 21.2 μM). The G-rich sequence G774-G790 within the IRES-A of hVEGF-A's 5′-UTR is critical for the translation initiation activity of IRES-A. hVEGF-IN-1 hinders BG4 from binding to the IRES-A RNA G-quadruplex in cells. hVEGF-IN-1 down-regulates the translation of hVEGF-A via the G-quadruplex within IRES-A mRNA. hVEGF-IN-1 reduces MDA-MB- 231 cell migration to about 25%.
In vivo	In tumor-bearing mice, hVEGF-IN-1 causes an average tumor volume of fewer than 300 mm3. In the presence of hVEGF-IN-1, the tumor weight reduces around 60.1% to a final weight of 0.18 g and no obvious change in body weight.
Cell Research	MDA-MB-231 cells are plated in the top chambers of 0.8 μm pore trans-wells in Opti-MEM reduced serum medium in the presence or absence of hVEGF-IN-1. Meanwhile, 600 μL of DMEM containing 10% fetal bovine serum (FBS) and 100 μM CoCl2 are added to the lower chambers. The cells are allowed to migrate for 24 h. At the end of the assay, the cells in the top chamber are removed, and the cells at the bottom of the filter are treated by adding 500 μL of DMEM containing 2.5 mg/mL MTT to each well. After incubating at 37 °C with 5% CO2 for 4 h, 500 μL of DMSO is added to each well and the plate is gently rotated for 10 min. Absorbance (570 nm) is measured using a microplate reader.
Animal Research	Mice are separated into three groups: negative control, compound 1-treated, and positive control (doxorubicin-treated). hVEGF-IN-1, doxorubicin, and saline are administered by intraperitoneal injection to athymic nude mice with human tumor xenografts established using MCF-7 breast cancer cells. Mice are injected intraperitoneal once a day for 20 days. Negative controls are injected with 150 μL of saline. The positive control group received doxorubicin by intraperitoneal injection at a dose of 1 mg/kg. hVEGF-IN-1 is similarly administered to mice at a dose of 7.5 mg/kg. After treating the animals for 20 days, the tumor tissues are collected and IHC assays are conducted using an anti-VEGF-A antibody[1].

Molecular Weight	581.75
Formula	C34H43N7O2
CAS No.	1637443-98-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 5.82 mg/mL (10 mM)

References and Literature

1. Discovery of Small Molecules for Repressing Cap-Independent Translation of Human VascularEndothelial Growth Factor (hVEGF) as Novel Antitumor Agents. J Med Chem. 2017 Jul 13;60(13):5306-5319.

Related compound libraries

This product is contained In the following compound libraries：

Tyrosine Kinase Inhibitor Library Inhibitor Library Kinase Inhibitor Library NO PAINS Compound Library Cytokine Inhibitor Library Anti-Prostate Cancer Compound Library Anti-Fibrosis Compound Library Anti-Pancreatic Cancer Compound Library Anti-Cancer Compound Library Bioactive Compound Library

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Keywords

hVEGF-IN-1 1637443-98-1 Angiogenesis Tyrosine Kinase/Adaptors VEGFR Inhibitor quinazoline IRES-A migration hVEGF IN 1 hVEGFIN1 tumor G-rich inhibit Vascular endothelial growth factor receptor VEGF-A inhibitor

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