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Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody

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Catalog No. TMAB-01528

Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody is a Rabbit antibody targeting Phospho-VEGFR2/KDR (Tyr1059). Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody

Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody

😃Good
Catalog No. TMAB-01528
Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody is a Rabbit antibody targeting Phospho-VEGFR2/KDR (Tyr1059). Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody is a Rabbit antibody targeting Phospho-VEGFR2/KDR (Tyr1059). Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Mouse,Human (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-VEGFR Polyclonal Antibody, Unconjugated (TMAB-01528) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: Huvec.
Primary Antibody (green line): Rabbit Anti-phospho-VEGF receptor 2 (Tyr1059) antibody (TMAB-01528)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
HUVEC (Hu) lysate at 30 μg
Primary: Anti-phospho-VEGF receptor 2 (Tyr1059) (TMAB-01528) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 147 kDa
Observed band size: 147 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationIsoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Note=Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus. Isoform 2: Secreted (Probable).Isoform 3: Secreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundVascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009].
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human VEGF receptor 2 around the phosphorylation site of Tyr1059
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Response to hypoxia,VEGF,Angiogenic growth factors,Growth factor receptors,Endothelial Cell Markers,VEGF Receptors,Endothelial Cells,Hypoxia,Host Virus Interaction,VEGF,Receptor Tyrosine Kinases,Endothelial Markers,Surface Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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