Anti-Phospho-VEGFR2/KDR (Tyr1175) Polyclonal Antibody is a Rabbit antibody targeting Phospho-VEGFR2/KDR (Tyr1175). Anti-Phospho-VEGFR2/KDR (Tyr1175) Polyclonal Antibody can be used in WB,FCM.

IgG

Mouse,Rat,Human (predicted:Dog,Pig,Cow,Horse,Rabbit)

1. Sample:
Lane 1: Placenta (Mouse) Lysate at 40 μg
Lane 2: Adrenal gland (Rat) Lysate at 40 μg
Primary: Anti-phospho-VEGFR2 (Tyr1175) (TMAB-01529) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 147 kDa
Observed band size: 220 kDa
2. Blank control: Huvec.
Primary Antibody (green line): Rabbit Anti-phospho-VEGFR2 (Tyr1175) antibody (TMAB-01529)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 2ug/test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human VEGFR2 around the phosphorylation site of Tyr1175

Human

Response to hypoxia,VEGF,Angiogenic growth factors,Growth factor receptors,Endothelial Cell Markers,VEGF Receptors,Endothelial Cells,Hypoxia,Host Virus Interaction,VEGF,Receptor Tyrosine Kinases,Endothelial Markers,Surface Molecules