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Anti-VEGFR2/KDR Polyclonal Antibody
😃Good
Catalog No. TMAB-01957
Anti-VEGFR2/KDR Polyclonal Antibody is a Rabbit antibody targeting VEGFR2/KDR. Anti-VEGFR2/KDR Polyclonal Antibody can be used in FCM.
Anti-VEGFR2/KDR Polyclonal Antibody
Anti-VEGFR2/KDR Polyclonal Antibody
😃Good
Catalog No. TMAB-01957
Anti-VEGFR2/KDR Polyclonal Antibody is a Rabbit antibody targeting VEGFR2/KDR. Anti-VEGFR2/KDR Polyclonal Antibody can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-VEGFR2/KDR Polyclonal Antibody is a Rabbit antibody targeting VEGFR2/KDR. Anti-VEGFR2/KDR Polyclonal Antibody can be used in FCM. |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow) |
| Verified Activity | Tissue/cell: Human esophageal cancer tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-VEGFR2 Polyclonal Antibody, Unconjugated (TMAB-01957) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. |
| Application | FCM |
| Recommended Dose | FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Note=Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus. Isoform 2: Secreted (Probable).Isoform 3: Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]. |
| Immunogen | KLH conjugated synthetic peptide: human VEGFR2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Response to hypoxia,VEGF,Angiogenic growth factors,Growth factor receptors,Endothelial Cell Markers,VEGF Receptors,Endothelial Cells,Hypoxia,Host Virus Interaction,VEGF,Receptor Tyrosine Kinases,Endothelial Markers,Surface Molecules |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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