Shopping Cart

Your shopping cart is currently empty

D-64131

Name: D-64131
Brand: TargetMol
SKU: T1753
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

🥰Excellent

Catalog No. T1753Cas No. 74588-78-6

D-64131 is a tubulin polymerization inhibitor with an IC50 value of 0.53 μM.

D-64131

Copy Product Info

🥰Excellent

Purity: 99.67%

Catalog No. T1753Cas No. 74588-78-6

D-64131 is a tubulin polymerization inhibitor with an IC50 value of 0.53 μM.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$29	In Stock	In Stock
10 mg	$43	In Stock	In Stock
25 mg	$95	In Stock	In Stock
50 mg	$173	In Stock	In Stock
100 mg	$270	In Stock	In Stock
200 mg	$398	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$35	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.67%

Appearance:Solid

Color:White

Resource Download

COA HPLC HNMR

Product Introduction

D-64131 AI Summary

D-64131 exhibits a broad spectrum of bioactivities, including inhibition of tubulin polymerization with an IC50 of 530.0 nM, and significant antiproliferative effects against various cell lines. It inhibits HeLa/KB cells with an IC50 of 68.0 nM, SK-OV-3 cells with an IC50 of 680.0 nM, U373 astrocytoma cells with an IC50 of 74.0 nM, and induces cell cycle arrest in U373 cells with an IC50 of 62.7 nM. Additionally, it affects beta-Tubulin GTPase activity and shows cytotoxicity against a panel of tumor cell lines with an IC70 of 0.22 µg/ml. In mice models, D-64131 impacts tumor growth, weight, and survival rates depending on the dosage and administration route. Furthermore, D-64131 exhibits antiviral properties, including the inhibition of SARS-CoV-2 induced cytotoxicity in several cell lines, with inhibition rates ranging from -0.11% to 1.31% at concentrations of 10-20 µM. It also shows inhibition of the SARS-CoV-2 3CL-Pro protease at 20 µM with inhibition percentages of 8.58% and 18.3%. The compound also inhibits the human HDAC6 enzyme with inhibition percentages over 100% in various assays and an IC50 of 771.5 nM. Its cytotoxicity against HepG2 cells is marked by an IC50 of 200.0 nM. This compound evidences notable antiviral and anticancer potential through its diverse bioactivity profile..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	D-64131 is a tubulin polymerization inhibitor with an IC50 value of 0.53 μM.
Targets&IC50	Tubulin polymerization:0.53 μM.
In vitro	D-64131 dose-dependently inhibited the polymerization of bovine brain microtubulin and the binding of [3H]colchicine to biotinylated αβ-microtubulin. After treatment with D-64131, many Y cells displayed abnormal microtubule structures, such as divided mitotic spindles or multiple spindle poles. Nuclear vesicles and abnormal nuclear structures typical of apoptotic cells were detected in many cells treated with D-64131.
In vivo	D-64131 dose-dependently inhibited the polymerization of bovine brain microtubulin and the binding of [3H]colchicine to biotinylated αβ-microtubulin. After treatment with D-64131, many Y cells displayed abnormal microtubule structures, such as divided mitotic spindles or multiple spindle poles. Nuclear vesicles and abnormal nuclear structures typical of apoptotic cells were detected in many cells treated with D-64131.

Chemical Properties

Molecular Weight	251.28
Formula	C₁₆H₁₃NO₂
Cas No.	74588-78-6
Smiles	COc1ccc2[nH]c(cc2c1)C(=O)c1ccccc1
Relative Density.	1.231 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 25.1 mg/mL (99.89 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.96 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.9796 mL	19.8981 mL	39.7962 mL	198.9812 mL
5 mM	0.7959 mL	3.9796 mL	7.9592 mL	39.7962 mL
10 mM	0.3980 mL	1.9898 mL	3.9796 mL	19.8981 mL
20 mM	0.1990 mL	0.9949 mL	1.9898 mL	9.9491 mL
50 mM	0.0796 mL	0.3980 mL	0.7959 mL	3.9796 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc