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4'-Demethylepipodophyllotoxin

Name: 4'-Demethylepipodophyllotoxin
Brand: TargetMol
SKU: T2937
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2937Cas No. 6559-91-7

Alias DMEP, 4'-O-demethylepipodophyllotoxin, 4'-DMEP, 4-Demethylepipodophyllotoxin

4'-Demethylepipodophyllotoxin (DMEP) is a potent inhibitor of microtubule assembly.

4'-Demethylepipodophyllotoxin

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Purity: 99.72%

Catalog No. T2937Alias DMEP, 4'-O-demethylepipodophyllotoxin, 4'-DMEP, 4-DemethylepipodophyllotoxinCas No. 6559-91-7

4'-Demethylepipodophyllotoxin (DMEP) is a potent inhibitor of microtubule assembly.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 g	$29	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.72%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

4'-Demethylepipodophyllotoxin AI Summary

4'-Demethylepipodophyllotoxin exhibits multiple bioactivities, including significant inhibition of human DNA topoisomerase II, with inhibition rates up to 60.0% and IC50 values related to cytotoxic activity against various cell lines, including H9 (70.0 nM), HeLa (980.0 nM), HL60 (820.0 nM), and multiple others, indicating its potential as an anti-cancer agent. It also shows potent in vitro cytotoxicity in KB cells with ED50 values ranging from 0.06 to 0.34 µg/ml and antiproliferative activities against various human cell lines such as HT-29, HT-1080, M21, and HaCaT with low nanomolar IC50 values. Additionally, 4'-Demethylepipodophyllotoxin demonstrates antiviral effects against HSV1 in human primary amnion cells and insecticidal activity against Mythimna separata larvae. Its log P value is 0.73, reflecting its partitioning characteristics, and it also shows inhibitory actions on TDP1, and exhibits various effects on enzyme activities and transport mechanisms, such as OATP1B1 and OATP1B3-transfected CHO cells. The compound has multifaceted applications in cytotoxicity, enzymatic inhibition, antiviral activity, and antiproliferative treatment..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	4'-Demethylepipodophyllotoxin (DMEP) is a potent inhibitor of microtubule assembly.
Targets&IC50	Microtubule:0.31μM(EC50)
Synonyms	DMEP, 4'-O-demethylepipodophyllotoxin, 4'-DMEP, 4-Demethylepipodophyllotoxin

Chemical Properties

Molecular Weight	400.38
Formula	C₂₁H₂₀O₈
Cas No.	6559-91-7
Smiles	O=C1[C@@]2([C@@H](C=3C([C@@H](O)[C@]2(CO1)[H])=CC4=C(C3)OCO4)C5=CC(OC)=C(O)C(OC)=C5)[H]
Relative Density.	1.446 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (124.88 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4976 mL	12.4881 mL	24.9763 mL	124.8814 mL
5 mM	0.4995 mL	2.4976 mL	4.9953 mL	24.9763 mL
10 mM	0.2498 mL	1.2488 mL	2.4976 mL	12.4881 mL
20 mM	0.1249 mL	0.6244 mL	1.2488 mL	6.2441 mL
50 mM	0.0500 mL	0.2498 mL	0.4995 mL	2.4976 mL
100 mM	0.0250 mL	0.1249 mL	0.2498 mL	1.2488 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc