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Bufalin

Name: Bufalin
Brand: TargetMol
SKU: T1719
Price: 48 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1719 Copy Product Info

Purity: 99.8%

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Bufalin is a natural product and a Na⁺/K⁺-ATPase inhibitor (IC50 ≈ 40–45 nM) with favorable cell permeability. By binding to α subunits such as ATP1A1, bufalin induces calcium overload and pyroptosis, and can be used for experimental research and therapeutic exploration in various cancers.

Cas No. 465-21-4

Pack Size	Price	USA Stock	Global Stock
5 mg	$48	In Stock	In Stock
10 mg	$80	In Stock	In Stock
25 mg	$133	In Stock	In Stock
50 mg	$197	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$53	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.8%

Color:White

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	Bufalin is a natural product and a Na⁺/K⁺-ATPase inhibitor (IC50 ≈ 40–45 nM) with favorable cell permeability. By binding to α subunits such as ATP1A1, bufalin induces calcium overload and pyroptosis, and can be used for experimental research and therapeutic exploration in various cancers.
Targets & IC50	Na+, K+-ATPase α1:42.5 nM (Kd), Na+, K+-ATPase α2:45 nM (Kd), Na+, K+-ATPase α3:40 nM (Kd)
In vitro	Methods: Human lung cancer cells A549 and H1299 were incubated with 40 ng/ml Bufalin for 24 hours. Results: Bufalin significantly inhibited cell viability, colony formation, and soft agar colony formation in a concentration-dependent manner.[1] Methods: Human colon cancer HCT-116 and SW620 cells were incubated with gradient concentrations of Bufalin for 48 hours, respectively. Results: Bufalin inhibited cell proliferation in a concentration-dependent manner, with IC₅₀ values of 12.823±1.792 nM and 26.303±2.498 nM, respectively.[2]
In vivo	Methods: A549 or H1299 cells were inoculated into nude mice. When tumor volume reached 220–250 mm³, Bufalin 1 mg/kg was administered by subcutaneous or intraperitoneal injection daily or every other day (with DMSO as the vehicle) for 25 consecutive days. Results: Tumor volume and weight in the Bufalin group were significantly lower than those in the control group. [1]

Chemical Properties

Molecular Weight	386.52
Formula	C₂₄H₃₄O₄
Cas No.	465-21-4
Smiles	[H][C@]12CC[C@]3([H])[C@]([H])(CC[C@]4(C)[C@H](CC[C@]34O)c3ccc(=O)oc3)[C@@]1(C)CC[C@H](O)C2
Relative Density.	1.226 g/cm3

Storage & Solubility Information

Storage

Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (258.72 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (10.35 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5872 mL	12.9359 mL	25.8719 mL	129.3594 mL
5 mM	0.5174 mL	2.5872 mL	5.1744 mL	25.8719 mL
10 mM	0.2587 mL	1.2936 mL	2.5872 mL	12.9359 mL
20 mM	0.1294 mL	0.6468 mL	1.2936 mL	6.4680 mL
50 mM	0.0517 mL	0.2587 mL	0.5174 mL	2.5872 mL
100 mM	0.0259 mL	0.1294 mL	0.2587 mL	1.2936 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc