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Bisindolylmaleimide VIII acetate

🥰Excellent
Catalog No. T10549Cas No. 138516-31-1
Alias Ro 31-7549 acetate, Bis VIII acetate

Bisindolylmaleimide VIII acetate (Ro 31-7549 acetate) is a potent and selective PKC inhibitor (IC50: 158 nM for rat brain PKC) with IC50 values of 53, 195, 163, 213, and 175 nM for PKC-α, PKC-βI, PKC-βII, PKC-γ, and PKC-ε, respectively.

Bisindolylmaleimide VIII acetate
Other Forms of Bisindolylmaleimide VIII acetate:
Bisindolylmaleimide VIIIT71125125313-65-7
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Bisindolylmaleimide VIII acetate

🥰Excellent
Purity: 99.27%
Catalog No. T10549Alias Ro 31-7549 acetate, Bis VIII acetateCas No. 138516-31-1
Bisindolylmaleimide VIII acetate (Ro 31-7549 acetate) is a potent and selective PKC inhibitor (IC50: 158 nM for rat brain PKC) with IC50 values of 53, 195, 163, 213, and 175 nM for PKC-α, PKC-βI, PKC-βII, PKC-γ, and PKC-ε, respectively.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
1 mg$39In StockIn Stock
5 mg$94In StockIn Stock
10 mg$149In StockIn Stock
25 mg$313In StockIn Stock
50 mg$455In StockIn Stock
100 mg$636In StockIn Stock
1 mL x 10 mM (in DMSO)$136In StockIn Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Purity:99.27%
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Product Introduction

Bioactivity
Description
Bisindolylmaleimide VIII acetate (Ro 31-7549 acetate) is a potent and selective PKC inhibitor (IC50: 158 nM for rat brain PKC) with IC50 values of 53, 195, 163, 213, and 175 nM for PKC-α, PKC-βI, PKC-βII, PKC-γ, and PKC-ε, respectively.
Targets&IC50
PKCγ:213 nM, PKCε:175 nM, PKC:158 nM(Rat Brain), PKCβ2:163 nM, PKCβ1:195 nM, PKCα:53 nM
In vitro
Bisindolylmaleimide VIII acetate facilitates Fas-mediated apoptosis and inhibits T cell-mediated autoimmune diseases. Bisindolylmaleimide VIII acetate (5 μM; 8, 12 hours) dramatically increases TRA-8-induced cell death in time-dependent and TRA-8 dose-dependent manners. Bisindolylmaleimide VIII acetate (5 μM; 6 hours) significantly decreases procaspase-8 at 4 h and completely disappears at 6 h after the combined treatment with TRA-8 [2].
In vivo
Bisindolylmaleimide VIII acetate (100 μg; i.p.; every other day for three doses) results in nearly complete tumor regression combined toTRA-8. The alone treatment with Bisindolylmaleimide VIII acetate does not induce significant tumor regression [2].
SynonymsRo 31-7549 acetate, Bis VIII acetate
Chemical Properties
Molecular Weight458.51
FormulaC26H26N4O4
Cas No.138516-31-1
SmilesCC(O)=O.Cn1cc(C2=C(C(=O)NC2=O)c2cn(CCCN)c3ccccc23)c2ccccc12
Relative Density.no data available
ColorRed
AppearanceSolid
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 55 mg/mL (119.95 mM), Sonication is recommended.
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.36 mM), Sonication is recommended.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.1810 mL10.9049 mL21.8098 mL109.0489 mL
5 mM0.4362 mL2.1810 mL4.3620 mL21.8098 mL
10 mM0.2181 mL1.0905 mL2.1810 mL10.9049 mL
20 mM0.1090 mL0.5452 mL1.0905 mL5.4524 mL
50 mM0.0436 mL0.2181 mL0.4362 mL2.1810 mL
100 mM0.0218 mL0.1090 mL0.2181 mL1.0905 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.
1 Enter information below:
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2 Enter the in vivo formulation:
% DMSO
%
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Ro 31-7549 AcetateRo 31-7549Rat Brain PKCProtein kinase CproteinPKC-εPKCεPKC-γPKCγPKC-βIIPKC-βIPKCβ2PKCβ1PKC-αPKCαPKCkinaseInhibitorinhibitFasdiseasescell-mediatedBisindolylmaleimide VIII acetateBisindolylmaleimide VIII AcetateBisindolylmaleimide VIIIBis VIII AcetateBis VIIIautoimmuneApoptosis
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