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Azosemide

Name: Azosemide
Brand: TargetMol
SKU: T10441
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T10441Cas No. 27589-33-9

Azosemide is a potent NKCC1 inhibitor (IC50s: 0.246 µM and 0.197 µM for hNKCC1A and NKCC1B).

Azosemide

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Purity: 99.84%

Catalog No. T10441Cas No. 27589-33-9

Azosemide is a potent NKCC1 inhibitor (IC50s: 0.246 µM and 0.197 µM for hNKCC1A and NKCC1B).

Pack Size	Price	Availability
25 mg	$41	In Stock
50 mg	$58	In Stock
100 mg	$89	In Stock
200 mg	$128	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock

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Batch Information

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Purity:99.84%

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Azosemide is a potent NKCC1 inhibitor (IC50s: 0.246 µM and 0.197 µM for hNKCC1A and NKCC1B).
Targets&IC50	NKCC1A (human):0.246 µM , NKCC1B:0.197 µM
In vitro	Azosemide inhibits the human sodium-potassium-chloride cotransporter variants hNKCC1B and hNKCC1A [1].
In vivo	Azosemide shows a shorter terminal half-life (50.9% decrease) and MRT (64.1% decrease), smaller AUC (81.9% decrease), faster CL (454% increase), CLNR (307% increase) and CLR (853% increase) for NARs [2].

Chemical Properties

Molecular Weight	370.84
Formula	C₁₂H₁₁ClN₆O₂S₂
Cas No.	27589-33-9
Smiles	NS(=O)(=O)c1cc(-c2nnn[nH]2)c(NCc2cccs2)cc1Cl
Relative Density.	1.6843 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 249 mg/mL (671.44 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6966 mL	13.4829 mL	26.9658 mL	134.8290 mL
5 mM	0.5393 mL	2.6966 mL	5.3932 mL	26.9658 mL
10 mM	0.2697 mL	1.3483 mL	2.6966 mL	13.4829 mL
20 mM	0.1348 mL	0.6741 mL	1.3483 mL	6.7415 mL
50 mM	0.0539 mL	0.2697 mL	0.5393 mL	2.6966 mL
100 mM	0.0270 mL	0.1348 mL	0.2697 mL	1.3483 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc