Bumetanide sodium

Name: Bumetanide sodium
Brand: TargetMol
SKU: T61713
Rating: 4.5 (1 reviews)

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Catalog No. T61713Cas No. 28434-74-4

Bumetanide sodium is a potent loop diuretic that acts as a blocker for the sodium-potassium-chloride cotransporter (NKCC). It selectively inhibits NKCC1 and NKCC2, with IC50 values of 0.68 μM and 4.0 μM for hNKCC1A and hNKCC2A, respectively [1] [2].

Bumetanide sodium

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Catalog No. T61713Cas No. 28434-74-4

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Product Introduction

Bioactivity

Description	Bumetanide sodium is a potent loop diuretic that acts as a blocker for the sodium-potassium-chloride cotransporter (NKCC). It selectively inhibits NKCC1 and NKCC2, with IC50 values of 0.68 μM and 4.0 μM for hNKCC1A and hNKCC2A, respectively [1] [2].
In vitro	Bumetanide sodium effectively inhibits the major human splice variants of NKCCs, specifically hNKCC1A and hNKCC2A. It demonstrates dose-dependent inhibition of ^86Rb^+ uptake in NKCC1A-expressing oocytes, ranging from 0.03 to 100 μM over 5 minutes. Additionally, Bumetanide sodium targets the NKCC2 isoform B in HEK-293 cells, achieving an IC50 value of 0.54 μM.
In vivo	Bumetanide sodium (7.6-30.4 mg/kg; i.v.) reduces the decline in apparent diffusion coefficients (ADC) ratios in the cortex and striatum by 40-67%, indicating decreased edema formation [3], and decreases infarct size [3]. It has varying half-lives—21.4 min, 53.8 min, and 137 min—after intravenous doses of 2 mg/kg, 8 mg/kg, and 20 mg/kg, respectively, in rats [4].

Chemical Properties

Molecular Weight	386.4
Formula	C₁₇H₁₉N₂NaO₅S
Cas No.	28434-74-4
Smiles	[Na+].CCCCNc1cc(cc(c1Oc1ccccc1)S(N)(=O)=O)C([O-])=O

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc