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UCF 101

Catalog No. T21919   CAS 313649-08-0

UCF 101 is a specific inhibitor of HtrA2 and reduces apoptosis in PC12 cells.

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UCF 101, CAS 313649-08-0
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Purity 98%
Biological Description
Chemical Properties
Storage & Solubility Information
Description UCF 101 is a specific inhibitor of HtrA2 and reduces apoptosis in PC12 cells.
In vitro PC12 cells treated with 60 μM 6-OHDA for 24 h had significantly decreased cell viability compared to that of controls. A low concentration (2.5 μM) of UCF 101 decreased the apoptosis rate of the PD cell model, but a high concentration (≥10 μM) increased the apoptosis rate, compared to that of controls. 6-OHDA upregulated the expression of HtrA2, α-syn, CHOP, Grp78 and active caspase-3 and reduced the levels of TH and XIAP[3].
In vivo Administration of UCF 101 shortly before reperfusion reduced postischemic myocardial apoptosis in a dose-dependent fashion, with the maximal protective effect observed at a dose of 1.5 μmol/kg. This dose of UCF 101 (ie, 1.5 μmol/kg) was used in all subsequent experiments. To further establish that inhibition of Omi/HtrA2 protease activity led to significant inhibition of apoptosis, the effect of UCF 101 on DNA ladder formation, a hallmark of apoptotic cell death, was determined. 30 minutes of ischemia followed by 3 hours of reperfusion resulted in significant DNA fragmentation, which was significantly reduced by treatment with UCF 101 before reperfusion[2].UCF 101 reduced the level of ERS and apoptosis in vivo. The ratio of p-GSK3β (Tyr216 to Ser9) increased in PD rats[3].
Molecular Weight N/A
CAS No. 313649-08-0

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 12.5 mg/mL (25.23 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

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