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Spermidine

Name: Spermidine
Brand: TargetMol
SKU: T4893
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4893Cas No. 124-20-9

Alias N-(4-Aminobutyl)-1,3-diaminopropane, N-(3-Aminopropyl)-1,4-diaminobutane

Spermidine (N-(3-Aminopropyl)-1,4-diaminobutane) inhibits NOS1 (nNOS). Spermidine binds and precipitates DNA and may be used for purification of DNA binding proteins. Spermidine activates PNK (polynucleotide kinase T4). Spermidine binds to and activates NMDA and has been shown to potentiate NMDA-induced currents in a concentration-dependent manner.

Spermidine

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Purity: 99.81%

Catalog No. T4893Alias N-(4-Aminobutyl)-1,3-diaminopropane, N-(3-Aminopropyl)-1,4-diaminobutaneCas No. 124-20-9

Pack Size	Price	USA Warehouse	Global Warehouse
50 mg	$31	In Stock	In Stock
100 mg	$44	In Stock	In Stock
200 mg	$52	In Stock	In Stock
500 mg	$66	-	In Stock
1 g	$81	-	In Stock
2 g	$97	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.81%

Color:White

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COA GC LCMS HNMR

Product Introduction

Spermidine AI Summary

**Spermidine** exhibits diverse bioactivities across several biological systems and cell lines. It is an inhibitor of polyamine transport, notably with a Ki value of 113.0 nM in MDA-MB-231 cells and a Ki of 4400.0 nM in T-47D human breast cancer cells, highlighting its potential as an anti-cancer agent. It has shown inhibitory effects on various enzymes and proteins, such as a Ki value of 2460.0 nM for binding to the polyamine transporter in L1210 cells and a binding constant (Log K = 5.4) to Ribonuclease S. The compound binds to DNA, with notable affinities like an IC50 of 98,000.0 nM for calf thymus DNA, exhibiting weak DNA binding overall. It shows minimal toxicity against F98 glioma cells (IC50 > 100,000.0 nM) and L1210 cell growth inhibition (IC50 > 100,000.0 nM). In terms of enzymatic activity, Spermidine inhibits human topoisomerase-2alpha (IC50 > 1,000,000.0 nM) and several carbonic anhydrase isoforms. It affects polyamine metabolism and exhibits differential modulation of enzymes such as ornithine decarboxylase, S-Adenosyl-methionine decarboxylase, and Spermidine/Spermine-Acetyltransferase in L1210 cells. It has moderate antibacterial activity (MIC99 > 512.0 µg/ml) and limited antitrypanosomal activity (IC50 = 3,300.0 nM) against Trypanosoma brucei gambiense and Leishmania donovani. In antiviral assays, it inhibits SARS-CoV-2 by 85.0% and shows specific interaction with the SARS-CoV-2 3CL-Pro protease, though the inhibition rate is modest (17.15% at 20 µM). Furthermore, Spermidine affects coagulation by inhibiting factor XIIIa-mediated crosslinking of fibronectin-collagen with an IC50 of 100,000.0 nM. The compound's overall biological significance encompasses its interactions with various biological targets and potential therapeutic applications in cancer, infectious diseases, and enzyme inhibition..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Spermidine (N-(3-Aminopropyl)-1,4-diaminobutane) inhibits NOS1 (nNOS). Spermidine binds and precipitates DNA and may be used for purification of DNA binding proteins. Spermidine activates PNK (polynucleotide kinase T4). Spermidine binds to and activates NMDA and has been shown to potentiate NMDA-induced currents in a concentration-dependent manner.
Targets&IC50	c-Src:106.4 ± 13.4nM (EC50), HeLa cells:121.3 μM
In vitro	METHODS: Human neuroblastoma cells SH-SY5Y were treated with Spermidine (0.05-10 µM) for 24-48 h. Mitochondrial metabolic activity was measured by MTT assay and ATP production assay. RESULTS: No effect of Spermidine was observed after 24 h. 0.1 and 1 µM Spermidine significantly increased cellular metabolic activity and ATP production after 48 h of treatment. The most effective concentration of Spermidine, 0.1 µM, induced an 8.2% increase in metabolic activity and a 4% increase in ATP production. [1] METHODS: The retinal pigment epithelial cell line ARPE-19 was treated with H2O2 (300 µM) and Spermidine (10 µM) for 24 h. Apoptosis was detected by Flow cytometry. RESULTS: 300 µM H2O2 significantly increased the frequency of annexin V-positive cells to approximately 25%, but Spermidine significantly inhibited this increase. The results suggest that Spermidine attenuates H2O2-mediated oxidative stress-induced apoptosis in ARPE-19 cells. [2]
In vivo	METHODS: To study the effects on diabetes, Spermidine (10 mM) was administered orally in water to non-obese diabetic (NOD) mice (type 1 diabetes mouse model) for thirty weeks. RESULTS: Treatment of NOD mice with Spermidine resulted in a higher incidence of diabetes, although pancreatic insulitis was not altered.Spermidine modulated pancreatic tissue polyamine levels and increased signs of autophagy.Spermidine resulted in an increase in the proportion of pro-inflammatory T-cells in the pancreatic lymph nodes (pLN) of diabetic mice. [3]
Synonyms	N-(4-Aminobutyl)-1,3-diaminopropane, N-(3-Aminopropyl)-1,4-diaminobutane

Chemical Properties

Molecular Weight	145.25
Formula	C₇H₁₉N₃
Cas No.	124-20-9
Smiles	NCCCCNCCCN
Relative Density.	1.00 g/cm3 at 20℃

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (1721.17 mM), Sonication is recommended.

H2O: 100 mg/mL (688.47 mM), Sonication is recommended.

In Vivo Formulation

5% DMSO+95% Saline: 1.67 mg/mL (11.5 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	6.8847 mL	34.4234 mL	68.8468 mL	344.2341 mL
5 mM	1.3769 mL	6.8847 mL	13.7694 mL	68.8468 mL
10 mM	0.6885 mL	3.4423 mL	6.8847 mL	34.4234 mL
20 mM	0.3442 mL	1.7212 mL	3.4423 mL	17.2117 mL
50 mM	0.1377 mL	0.6885 mL	1.3769 mL	6.8847 mL
100 mM	0.0688 mL	0.3442 mL	0.6885 mL	3.4423 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc