Salidroside is a bioactive phenolic glycoside compound isolated from Rhodiola rosea and is a prolyl endopeptidase inhibitor. Salidroside can alleviate cachexia symptoms in a tumor cachexia mouse model by activating mTOR signaling and protect dopaminergic neurons by enhancing PINK1/Parkin-mediated mitochondrial autophagy. Salidroside can also inhibit the growth of cancer cells by regulating the CDK4-cyclin D1 pathway to block the G1 phase and/or regulating the Cdc2-cyclin B1 pathway to block the G2 phase.

METHODS: Min6 cells were co-treated with salidroside (Rhodioloside) (50 μM, 3 days), glucose or H2O2, and the changes in protein levels in β cells were detected by western blot.
RESULTS Salidroside downregulated the expression of NOX2 and inhibited the subsequent activation of JNK and caspase 3, thereby preventing β cell death. [4]
METHODS: SH-SY5Y cells were treated with Salidroside (Rhodioloside) (25-100 μM, 24 hours) and then exposed to MPP+ (500 μM, 24 hours). Cell viability was determined by MTT assay, cell apoptosis was analyzed by flow cytometry, and cell morphology was evaluated by Hoechst staining.
RESULTS Salidroside concentration-dependently prevented the decrease in cell viability induced by MPP+; Salidroside concentration-dependently significantly reduced the number of MPP+-treated annexin V/PI-stained cells; in Hoechst staining, Salidroside significantly inhibited the MPP+-induced increase in chromatin condensation, hyperfluorescence, and nuclear fragmentation in SH-SY5Y cells. [5]
METHODS: siDJ-1 transfected H-SY5Y cells were treated with Salidroside (25-100 μM, 24 hours), and then treated with MPP+ (500 μM, 24 hours). , the mRNA expression of DJ-1, Nrf2, GCLc, SOD1 and SOD2 was determined and statistically analyzed.
RESULTS Silencing of DJ-1 significantly inhibited the Salidroside-induced increase in mRNA and protein levels (DJ-1, Nrf2, GCLc, SOD1 and SOD2) in MPP+-treated SH-SY5Y cells; silencing DJ-1 also significantly Significantly inhibited the Salidroside-induced decrease in ROS levels and increase in GSH levels in MPP+-treated cells. [5]

METHODS: Salidroside (Rhodioloside) (100 mg, oral, once a day. 5 weeks) was used to treat db/db mice after 10 weeks of HFD, and its effect on db/db mice with prediabetes at 4 weeks was observed; after 5 weeks of treatment , conduct OGTT experiment
RESULTS Salidroside could not significantly alleviate the elevated blood glucose in db/db mice within the first 15 days; Salidroside protected db/db mice from severe hyperglycemia after 21 days of treatment; Salidroside-treated db/db mice were less Rats' tolerance to glucose was significantly improved. [4]