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Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody

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Catalog No. TMAB-01458

Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody is a Rabbit antibody targeting Phospho-mTOR (Ser2448). Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody

Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody

😃Good
Catalog No. TMAB-01458
Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody is a Rabbit antibody targeting Phospho-mTOR (Ser2448). Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody is a Rabbit antibody targeting Phospho-mTOR (Ser2448). Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-mTOR (Ser2448) Polyclonal Antibody, Unconjugated (TMAB-01458) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Image provided by Independent Validation (badge 029744). Histogram of serum-stimulated (positive control) and serum-starved (negative control) MCF-7 cells stained with Rabbit Anti-mTOR (Ser2448) Polyclonal Antibody (orange)(TMAB-01458 at 1:100), isotype control antibody (green), secondary antibody only (blue) and unstained (red). Strong signal is observed in both positive and negative controls. No change in expression is noted upon serum stimulation, as has been demonstrated in the literature. There is no evidence to support the antigen recognizing a phosphorylated version of the target.
3. Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-phospho-mTOR Polyclonal Antibody, Unconjugated (TMAB-01458) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-phospho-mTOR Polyclonal Antibody, Unconjugated (TMAB-01458) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-Phospho-mTOR (Ser2448) antibody (TMAB-01458), Dilution: 1 μg/10^6 cells.
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test.
6. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-mTOR (Ser2448)) Polyclonal Antibody, Unconjugated (TMAB-01458) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-mTOR (Ser2448)) Polyclonal Antibody, Unconjugated (TMAB-01458) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome. Cytoplasm (By similarity). Nucleus, PML body (By similarity). Note=Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundmTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 289-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human mTOR around the phosphorylation site of Ser2448
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cell cycle inhibitors,Transcription factors/regulators,Cell Cycle Inhibitors,DNA Damage Recognition,Growth factors and hormones ELISA kits,Obesity
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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