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SANT-1

Catalog No. T2450 CAS 304909-07-7

Synonyms: SANT 1, SANT1

SANT-1 directly binds to Smoothened (Smo) receptor (Kd: 1.2 nM) and inhibits Smo agonist effects (IC50: 20 nM).

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SANT-1, CAS 304909-07-7

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	SANT-1 directly binds to Smoothened (Smo) receptor (Kd: 1.2 nM) and inhibits Smo agonist effects (IC50: 20 nM).
Targets&IC50	Smoothened receptor:1.2 Nm
Kinase Assay	CK1 kinase assay: All protein kinase assays (25 μL) are carried out at room temperature (21℃). Assays are performed for 40 min using a Biomek 2000 Laboratory Automation Workstation in a 96-well format. The concentrations of magnesium acetate and [γ-33P]ATP (800 cpm/pmol) in the assays are 10 mM and 0.1 mM, respectively. Assays are initiated with MgATP and stopped by the addition of 5 μL of 0.5 M orthophosphoric acid. Aliquots are then spotted on to P30 ?ltermats, washed four times in 75 mM phosphoric acid to remove ATP, once in methanol, then dried and counted for radioactivity. CK1 δ (5-20 m-units), diluted in 20 mM Hepes, pH 7.5, 0.15 M NaCl, 0.1 mM EGTA, 0.1% (v/v) Triton X-100, 5 mM dithiothreitol, 50% (v/v) glycerol, is assayed against the peptide RRKDLHDDEEDEAMSITA in an incubation containing 20 mM Hepes, pH 7.5, 0.15 M NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100 and 0.5 mM substrate peptide.

Synonyms	SANT 1, SANT1
Molecular Weight	373.504
Formula	C23H27N5
CAS No.	304909-07-7

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 18.7 mg/mL (50 mM)

Ethanol: 9.3 mg/mL (25 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. James K. Chen, et al. Proc Natl Acad Sci U S A, 2002, 99(22), 14071-14076. 2. Wilson CW, et al. PLoS One. 2009;4(4):e5182. 3. Chun SG, et al. Cancer Biol Ther, 2009, 8(14), 1328-1339.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library GPCR Compound Library Anti-Obesity Compound Library Anti-Pancreatic Cancer Compound Library Wnt/Hedgehog/Notch Compound Library Anti-Breast Cancer Compound Library Stem Cell Differentiation Compound Library Neuronal Signaling Compound Library Target-Focused Phenotypic Screening Library Anti-Aging Compound Library

Related Products

Related compounds with same targets

Glasdegib HhAntag Triparanol SMANT hydrochloride MRT-10 GSA-10 HPI 1 TAK-441

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

SANT-1 304909-07-7 G蛋白偶联受体干细胞 Hedgehog/Smoothened Smo SANT1 Smoothened Hedgehog Inhibitor SANT 1 inhibit inhibitor

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