Powder: -20°C for 3 years
In solvent: -80°C for 2 years
SANT-1 directly binds to Smoothened (Smo) receptor (Kd: 1.2 nM) and inhibits Smo agonist effects (IC50: 20 nM).
Description | SANT-1 directly binds to Smoothened (Smo) receptor (Kd: 1.2 nM) and inhibits Smo agonist effects (IC50: 20 nM). |
Targets&IC50 | Smoothened receptor:1.2 Nm |
Kinase Assay | CK1 kinase assay: All protein kinase assays (25 μL) are carried out at room temperature (21℃). Assays are performed for 40 min using a Biomek 2000 Laboratory Automation Workstation in a 96-well format. The concentrations of magnesium acetate and [γ-33P]ATP (800 cpm/pmol) in the assays are 10 mM and 0.1 mM, respectively. Assays are initiated with MgATP and stopped by the addition of 5 μL of 0.5 M orthophosphoric acid. Aliquots are then spotted on to P30 ?ltermats, washed four times in 75 mM phosphoric acid to remove ATP, once in methanol, then dried and counted for radioactivity. CK1 δ (5-20 m-units), diluted in 20 mM Hepes, pH 7.5, 0.15 M NaCl, 0.1 mM EGTA, 0.1% (v/v) Triton X-100, 5 mM dithiothreitol, 50% (v/v) glycerol, is assayed against the peptide RRKDLHDDEEDEAMSITA in an incubation containing 20 mM Hepes, pH 7.5, 0.15 M NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100 and 0.5 mM substrate peptide. |
Synonyms | SANT 1, SANT1 |
Molecular Weight | 373.504 |
Formula | C23H27N5 |
CAS No. | 304909-07-7 |
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
DMSO: 18.7 mg/mL (50 mM)
Ethanol: 9.3 mg/mL (25 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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SANT-1 304909-07-7 G蛋白偶联受体 干细胞 Hedgehog/Smoothened Smo SANT1 Smoothened Hedgehog Inhibitor SANT 1 inhibit inhibitor