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Rhosin hydrochloride

Name: Rhosin hydrochloride
Brand: TargetMol
SKU: T16745
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T16745Cas No. 1281870-42-5

Rhosin hydrochloride is a specific inhibitor of the RhoA subfamily Rho GTPases and inhibits the RhoA-GEF interaction. Rhosin hydrochloride can significantly induce cell apoptosis without affecting cell cycle progression. [1]

Rhosin hydrochloride

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Purity: 98.92%

Catalog No. T16745Cas No. 1281870-42-5

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$34	In Stock	In Stock
5 mg	$53	In Stock	In Stock
10 mg	$98	In Stock	In Stock
25 mg	$228	In Stock	In Stock
50 mg	$428	In Stock	In Stock
100 mg	$773	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$58	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.92%

Appearance:Solid

Color:Yellow

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	Rhosin hydrochloride is a specific inhibitor of the RhoA subfamily Rho GTPases and inhibits the RhoA-GEF interaction. Rhosin hydrochloride can significantly induce cell apoptosis without affecting cell cycle progression. [1]
Targets&IC50	RhoA:(kd)0.4 uM
In vitro	METHODS: To investigate the cytotoxic effects of Rhosin hydrochloride on B16BL6 and 4T1 cells, cell viability was assessed by treating cells with 1-100 μM Rhosin hydrochloride. RESULTS Rhosin hydrochloride at a concentration of 100 μM induced cell death in B16BL6 and 4T1 cells. [1]
In vivo	METHODS: B16BL6 cells and 4T1 cells were injected into the tail vein of syngeneic C57BL/6J mice and Balb/c mice. Mice were treated with 10 or 30 mg/kg of Rhosin hydrochloride daily from day 1 to day 14. RESULTS The number of lung metastatic nodules in B16BL6 and 4T1 cells was reduced in a dose-dependent manner after administration of Rhosin hydrochloride, and Rhosin hydrochloride inhibited the metastasis of 4T1-luc tumor cells to the lung region, which could be confirmed by the reduction of photon flux. [1]

Chemical Properties

Molecular Weight	431.318
Formula	C₂₀H₂₀Cl₂N₆O
Cas No.	1281870-42-5
Smiles	Cl.N[C@H](Cc1c[nH]c2ccccc12)C(=O)N\N=C\c1ccc2nccnc2c1
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 49 mg/mL (113.61 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (9.27 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3185 mL	11.5924 mL	23.1847 mL	115.9237 mL
5 mM	0.4637 mL	2.3185 mL	4.6369 mL	23.1847 mL
10 mM	0.2318 mL	1.1592 mL	2.3185 mL	11.5924 mL
20 mM	0.1159 mL	0.5796 mL	1.1592 mL	5.7962 mL
50 mM	0.0464 mL	0.2318 mL	0.4637 mL	2.3185 mL
100 mM	0.0232 mL	0.1159 mL	0.2318 mL	1.1592 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc