Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Micheliolide(MCL) is a sesquiterpene lactone which inhibits various inflammatory response.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 39.00 | |
5 mg | In stock | $ 122.00 | |
10 mg | In stock | $ 197.00 | |
25 mg | In stock | $ 333.00 | |
50 mg | In stock | $ 497.00 | |
100 mg | In stock | $ 693.00 | |
500 mg | In stock | $ 1,450.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 133.00 |
Description | Micheliolide(MCL) is a sesquiterpene lactone which inhibits various inflammatory response. |
In vitro | Treatment of BV2 cells with Micheliolide(MCL) significantly repressed LPS-stimulated nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, as well as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and nitric oxide (NO) induction.?MCL also attenuated mRNA levels of multiple pro-inflammatory cytokines and mediators such as iNOS, COX-2, TNF-α, IL-6 and IL-1β.?Mechanistic studies revealed that MCL suppressed LPS-stimulated the activation of IκBα/NF-κB pathway and Akt pathway.?Moreover, MCL inhibited LPS-induced the activition of c-Jun N-terminal kinase (JNK), p38 MAPK kinase, and extracellular signal-regulated kinases 1/2 (ERK1/2).?Meanwhile, MCL markedly promoted antioxidant protein heme oxygenase-1 (HO-1) expression by enhancing NF-E2-related factor 2 (Nrf2) activity.?Together, ?MCL may serve as a neuroprotective agent in neuroinflammation-related neurodegenerative disorders. |
Cell Research | BV2 cells were pretreated with the indicated concentrations of Micheliolide?1 h prior to stimulation with LPS (1 μg/ml) for 6 h and total RNA was extracted using RaPure Total?RNA Kit.Reverse transcription was performed using RevertAid First Strand cDNA Synthesis Kit.?Real-time PCR analysis was performed using SYBR Select Master Mix ?in conjunction with an ABI Prism 7500 Sequence Detection System with the expression of β-actin as the internal control.?The data were analyzed using the ΔΔCT method.?The primers for iNOS, COX-2, TNF-α, IL-1β, IL-6 and β-actin were described as below. |
Source |
Molecular Weight | 248.32 |
Formula | C15H20O3 |
CAS No. | 68370-47-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 49 mg/mL (197.32 mM)
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Micheliolide 68370-47-8 Immunology/Inflammation Neuroscience NF-Κb COX NF-κB NOS Nuclear factor-κB inhibit Nuclear factor-kappaB Inhibitor inhibitor