Powder: -20°C for 3 years
In solvent: -80°C for 2 years
LY2886721, a BACE inhibitor, is used for the therapy of Alzheimer's Disease.
Description | LY2886721, a BACE inhibitor, is used for the therapy of Alzheimer's Disease. |
Targets&IC50 | BACE2:10.2 nM, BACE1:20.3 nM |
In vitro | LY2886721 is an oral, small molecule of β-site amyloid protein cleaving enzyme (BACE) inhibitor with the potential to inhibit the synthesis of β-amyloid and thereby to slow the Clinicalal progression of AD. [1] LY2886721 can also targetγ-secretase to nhibit the synthesis of β-amyloid. [2] |
Kinase Assay | The CTLs and NK cells (0.8×106/mL) are treated with the inhibitors L1 (10-20 μM) or Aloxistatin (20-30 μM) for 24 hr at 37°C in 24-well plates. Cells are then used in 51Cr-release assays or are lysed to examine perforin in Western blots. The inhibitor is also added at the same concentration during the 4 hr reactions in some 51Cr-release assays, as indicated. Cell lysates are prepared using NP-40 lysis buffer (25 mM HEPES, 250 mM NaCl, 2.5 mM ethylenediaminetetraacetic acid, 0.1% volume/volume Nonidet P-40) and total protein concentration is determined using the Bradford assay. Equal amounts of protein are loaded and resolved on 8% SDS-PAGE gels. Human or mouse perforin is detected using the appropriate antibodies as indicated. Anti-actin antibody is used as a loading control[2]. |
Molecular Weight | 390.41 |
Formula | C18H16F2N4O2S |
CAS No. | 1262036-50-9 |
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
Ethanol: <1 mg/mL
H2O: <1 mg/mL
DMSO: 9 mg/mL (23.05 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
LY2886721 1262036-50-9 神经科学 BACE Beta-Secretase BACE2 Inhibitor Beta-secretase LY 2886721 LY-2886721 BBB Alzheimer's BACE1 sAPPβ C99 inhibit disease β-Secretase inhibitor