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Gramine

Catalog No. T6519 CAS 87-52-5

Synonyms: NSC 16892, Donaxine

Gramine, a natural indole alkaloid, present in several plant species, acts as an active adiponectin receptor (AdipoR) agonist, with IC50s of 3.2 and 4.2 μM for AdipoR2 and AdipoR1, respectively. Gramine is also a human and mouse β2-Adrenergic receptor (β2-AR) agonist. Gramine has anti-tumor, anti-viral and anti-inflammatory properties.

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Gramine, CAS 87-52-5

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Gramine, a natural indole alkaloid, present in several plant species, acts as an active adiponectin receptor (AdipoR) agonist, with IC50s of 3.2 and 4.2 μM for AdipoR2 and AdipoR1, respectively. Gramine is also a human and mouse β2-Adrenergic receptor (β2-AR) agonist. Gramine has anti-tumor, anti-viral and anti-inflammatory properties.
Targets&IC50	AdipoR1:4.2 μM, AdipoR2:3.2 μM
Kinase Assay	Assay of protein kinase C: Protein kinase C is arrayed by measuring 32PI transferred from [gamma-32PI] ATP to lysine-rich histone type Ill-s. The reaction mixture (80 μL) contained 50 mM Tris-HCI. pH 7.4, 100 μM CaCl2, 10 mM MgCI2, 37.5 μL/mL histone type Ill-s, 10 μM [gamma-32PI] ATP (1250 cpm/pmol), 31 μM bovine brain phosphatidylserine and 0.5 μM 1,2 sn-dioleylglycerol. Fifteen μL of purified PKC (final concentration in assay 0.38 μg/mL) is added to the incubation mixture. After 10 min at 30°C, the reaction is stopped by addition of 30 μL of casein 30 mg/mL and 0.9 ml of 12% trichloroacetic acid. The acid precipitable material is collected by centrifugation, dissolved in 1N NaOH (100μL) and precipitated again with 1 ml of 12% trichloroacetic acid. The pellet is dissolved in 1N NaOH (100μL) and 32P incorporation is measured by scintillation counting in Aquasol.

Synonyms	NSC 16892, Donaxine
Molecular Weight	174.24
Formula	C11H14N2
CAS No.	87-52-5

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

Ethanol: 33 mg/mL (189.4 mM)

H2O: <1 mg/mL

DMSO: 33 mg/mL (189.4 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Liu SL, Wang WW, Liu F, Li HY, Liu JS, Yang WD.Bull Environ Contam Toxicol. 2016 Apr;96(4):540-3. 2. Wei Y, Shi L, Wang K, Liu M, Yang Q, Yang Z, Ke S.Molecules. 2014 Jun 27;19(7):8949-64.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Gramine 87-52-5 GPCR/G Protein Microbiology/Virology Neuroscience Adrenergic Receptor Adiponectin receptor Reverse Transcriptase NSC 16892 AdipoRs Inhibitor Adiponectin Receptor Beta Receptor inhibit Donaxine inhibitor

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