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EF-5

Catalog No. T15202 CAS 152721-37-4

Synonyms: 2-Nitroimidazole, EF5

EF-5 (2-Nitroimidazole) is a agent of hypoxia labeling. It is used to identify hypoxia in cells.

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EF-5, CAS 152721-37-4

Pack Size	Availability	Price/USD
1 mg	In stock	$ 31.00
5 mg	In stock	$ 72.00
10 mg	In stock	$ 122.00
25 mg	In stock	$ 247.00
50 mg	In stock	$ 397.00
100 mg	In stock	$ 589.00
1 mL * 10 mM (in DMSO)	In stock	$ 79.00

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Purity: 98.26%

Biological Description

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Description	EF-5 (2-Nitroimidazole) is a agent of hypoxia labeling. It is used to identify hypoxia in cells.
In vitro	EF-5 binding and metabolic reduction of the prodrugs are highly correlated in a panel of 14 hypoxic tumor cell lines. Overexpression of CYPOR causes similar 2- to 4-fold increases in EF-5 binding and metabolic reduction of tirapazamine and CEN-209 in SiHa and HCT116 cell lines. Which is similar enhancement of γH2AX formation.[1].
In vivo	EF-5 binding is a promising stratification biomarker for benzotriazine-N-oxide bioreductive prodrugs. Binding and detection using a monoclonal antibody in 9L gliomas is specific and oxygen dependent, following intravenous injection of EF-5. Modification of tumor hypoxia causes similar changes to the bioreductive activation of both agents, resulting in a strong correlation between EF-5 binding and CEN209-induced DNA damage at the individual tumor level[1]. Detection of binding using fluorescence microscopy can be performed on frozen tissues; tissue sections can be counterstained with haematoxylin and eosin for light microscopic analysis. The distribution of hypoxia in a tumor can be inferred by examining individual tumor cells using flow cytometric techniques[2].

Synonyms	2-Nitroimidazole, EF5
Molecular Weight	302.16
Formula	C8H7F5N4O3
CAS No.	152721-37-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 125 mg/mL (413.69 mM), Sonication is recommended.

References and Literature

1. Wang J, et al. The 2-nitroimidazole EF5 is a biomarker for oxidoreductases that activate the bioreductive prodrug CEN-209 under hypoxia. Clin Cancer Res. 2012 Mar 15;18(6):1684-95. 2. Evans SM, et al. Identification of hypoxia in cells and tissues of epigastric 9L rat glioma using EF5 [2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide]. Br J Cancer. 1995 Oct;72(4):875-82.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Drug Repurposing Compound Library Anti-Cancer Clinical Compound Library Anti-Cancer Drug Library NO PAINS Compound Library Human Metabolite Library Anti-Cancer Compound Library Clinical Compound Library ReFRAME Related Library Bioactive Compounds Library Max

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Keywords

EF-5 152721-37-4 Others 2-Nitroimidazole inhibit EF 5 EF5 Inhibitor inhibitor

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