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Daunorubicin hydrochloride

Catalog No. T1511 CAS 23541-50-6

Synonyms: Daunomycin HCl, RP-13057 Hydrochloride, Rubidomycin hydrochloride, Daunorubicin HCl, Daunomycin

Daunorubicin hydrochloride (Rubidomycin hydrochloride), an anthracycline aminoglycoside antineoplastic, inhibits DNA replication and repair and RNA and protein synthesis.

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Daunorubicin hydrochloride Chemical Structure

Daunorubicin hydrochloride, CAS 23541-50-6

Pack Size	Availability	Price/USD
5 mg	In stock	$ 31.00
10 mg	In stock	$ 51.00
25 mg	In stock	$ 84.00
50 mg	In stock	$ 122.00
100 mg	In stock	$ 197.00
200 mg	In stock	$ 313.00
500 mg	In stock	$ 492.00
1 mL * 10 mM (in DMSO)	In stock	$ 57.00

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Purity: 99.53%

Purity: 98.60%

Purity: 96.21%

Biological Description

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Storage & Solubility Information

Description	Daunorubicin hydrochloride (Rubidomycin hydrochloride), an anthracycline aminoglycoside antineoplastic, inhibits DNA replication and repair and RNA and protein synthesis.
Targets&IC50	DNA synthesis:20 nM(Ki)
In vitro	At drug concentrations that reflect the peak plasma concentration after Daunorubicin administration, the primary mechanism is likely to be through interaction with topoisomerase II, which may be a primary triggering event for growth arrest and/or cell killing through a signalling pathway leading to apoptosis, at least in leukemic cells and thymocytes. The quinone structure permits daunorubicin to act as electron acceptors in reactions mediated by oxoreductive enzymes including cytochrome P450 reductase, NADH dehydrogenase, and xanthine oxidase. At Daunorubicin concentrations exceeding approximately 2–4 μM, free radical mediated toxicity and DNA cross-linking may become evident. Daunorubicin inhibits both DNA and RNA syntheses in HeLa cells over a concentration range of 0.2 through 2 μM. Daunorubicin inhibits both DNA syntheses in Ehrlich ascites tumor cells over a concentration range of 4 μM. Daunorubic triggers apoptosis at concentrations of 0.5 and 1 μM in either HL-60 or U-937 human leukemic cells. [1] Daunorubicin stimulates ceramide elevation and apoptosis in P388 and U937 cells through de novo synthesis via activation of the enzyme ceramide synthase. [2] Daunorubicin dose-dependently increases the phosphatidylserine exposure and consequent procoagulant activity of human umbilical vein endothelial cells. Daunorubicin (0.2 mM) significantly enhances the release of endothelial microparticles which are highly procoagulant in human umbilical vein endothelial cells. [3]
In vivo	Urinary protein excretion, serum creatinine, and blood urea nitrogen (BUN) level are significantly increased in group Daunorubicin (3 mg/kg, i.v.) compared with those in group Control. Administration of Daunorubicin (DNR) causes a significant increase in malondialdehyde (MDA) level in renal tissue compared with that in the control group[5].
Cell Research	Daunorubicin (Dnr) is prepared in PBS[2]. The chemosensitivity to Daunorubicin is assessed using the MTT assay. In brief, the 96 well plates are set up with cells at the initial density of 2×105 cells/mL and are incubated at 37°C for 72 h in an atmosphere of 5% CO2 in the absence and presence of nine different concentrations of Daunorubicin (Dnr) or Dox ranging from 1.90 to 0.007 μM in triplicate. After incubation, 10 μL of MTT solution (5 mg/mL tetrazolium salt) is added to each well and the plates are incubated for a further 4 h at 37°C. The formazan salt crystals are dissolved by adding 100 μL 10% SDS in 10 mM HCl solution and incubating over night at 37°C. The absorbance is measured at 540 nm with a reference at 650 nm by a 96-well enzyme-linked immunosorbent assay (ELISA) plate reader. Chemosensitivity is expressed as the IC50, which is the concentration of drug causing 50% cell survival compare to control cells grown without drug. Calculations are carried out using Microsoft Excel[2].

Synonyms	Daunomycin HCl, RP-13057 Hydrochloride, Rubidomycin hydrochloride, Daunorubicin HCl, Daunomycin
Molecular Weight	563.99
Formula	C27H29NO10·HCl
CAS No.	23541-50-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 92 mg/mL (163.1 mM)

DMSO: 93 mg/mL (164.9 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Gewirtz DA, et al. Biochem Pharmacol, 1999, 57(7), 727-741. 2. Bose R, et al. Cell, 1995, 82(3), 405-414. 3. Fu Y, et al. Thromb Haemost, 2010, 104(6), 1235-1241. 4. Tardi P, et al. Leuk Res, 2009, 33(1), 129-139. 5. Arozal W, et al. Telmisartan prevents the progression of renal injury in daunorubicin rats with the alteration of angiotensin II and endothelin-1 receptor expression associated with its PPAR-γ agonist actions. Toxicology. 2011 Jan 11;279(1-3):91-9.

Citations

1. Wang B, He B S, Ruan X L, et al. An integrated microfluidics platform with high-throughput single-cell cloning array and concentration gradient generator for efficient cancer drug effect screening. Military Medical Research. 2022, 9(1): 1-17. 2. Idris N, Leong K H, Wong E H, et al.Unveiling synergism of polymyxin B with chloramphenicol derivatives against multidrug-resistant (MDR) Klebsiella pneumoniae.The Journal of Antibiotics.2023: 1-9.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Clinical Compound Library Drug Repurposing Compound Library Microbial Natural Product Library Anti-Cancer Active Compound Library Anti-Cancer Approved Drug Library EMA Approved Drug Library Anti-Cancer Drug Library Anti-Viral Compound Library Approved Drug Library Apoptosis Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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Keywords

Daunorubicin hydrochloride 23541-50-6 Apoptosis Autophagy Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology Antibacterial Antibiotic DNA/RNA Synthesis Topoisomerase RP13057 pancreatic carcinoma leukemia K562 cells L3.6 cell Ehrlich ascites tumor chamomile RP 13057 Hydrochloride RP-13057 Inhibitor Daunomycin HCl RP13057 Hydrochloride Rubidomycin ADC Payload Anti-tumor Rubidomycin Hydrochloride inhibit Molt-4 cell ADC Cytotoxin Daunorubicin cytotoxin RP 13057 RP-13057 Hydrochloride Bacterial Rubidomycin hydrochloride Daunorubicin Hydrochloride Daunorubicin HCl Daunomycin inhibitor

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