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Bicuculline

Name: Bicuculline
Brand: TargetMol
SKU: T2850
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2850Cas No. 485-49-4

Alias d-Bicuculline, (+)-Bicuculline

Bicuculline is an alkaloid extracted from Corydalis decumbens, acting as a competitive antagonist of the neurotransmitter GABAA receptor (IC50 = 2 μM). It also blocks Ca2+-activated potassium (SK) channels and inhibits slow afterhyperpolarization (slow AHP). Bicuculline has anticonvulsant effects and is commonly used to establish mouse seizure models.

Bicuculline

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Purity: 99.99%

Catalog No. T2850Alias d-Bicuculline, (+)-BicucullineCas No. 485-49-4

Pack Size	Price	Availability
20 mg	$38	In Stock
50 mg	$63	In Stock
1 mL x 10 mM (in DMSO)	$50	In Stock

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Purity:99.99%

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	Bicuculline is an alkaloid extracted from Corydalis decumbens, acting as a competitive antagonist of the neurotransmitter GABAA receptor (IC50 = 2 μM). It also blocks Ca2+-activated potassium (SK) channels and inhibits slow afterhyperpolarization (slow AHP). Bicuculline has anticonvulsant effects and is commonly used to establish mouse seizure models.
Targets&IC50	GABAA receptor:2 μM
In vivo	(+)-Bicuculline, at concentrations of 1/3 μM, increased the EC50 of gamma-aminobutyric acid (GABA) by 1.6 times (41.0-67.0 μM) and by 3.6 times (36.1-129.0 μM), respectively. This compound also dose-dependently inhibited the Cl- conductance induced by GABA (40 μM) in the range of 1-100 μM. In addition, (+)-Bicuculline inhibited the agonistic effects of GABA (40 μM) on the α1β2γ2L receptor, functioning as an antagonist of the α1β2γ2L GABAA receptor. This action caused a parallel shift in the concentration-effect curve of GABA without affecting its maximum response. Furthermore, (+)-Bicuculline also exhibited inhibitory effects on Ca2+-activated potassium channels.
Synonyms	d-Bicuculline, (+)-Bicuculline

Chemical Properties

Molecular Weight	367.35
Formula	C₂₀H₁₇NO₆
Cas No.	485-49-4
Smiles	[H][C@]1(OC(=O)c2c3OCOc3ccc12)[C@@]1([H])N(C)CCc2cc3OCOc3cc12
Relative Density.	1.3694 g/cm3 (Estimated)
Color	Yellow
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 16.67 mg/mL (45.37 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.44 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7222 mL	13.6110 mL	27.2220 mL	136.1100 mL
5 mM	0.5444 mL	2.7222 mL	5.4444 mL	27.2220 mL
10 mM	0.2722 mL	1.3611 mL	2.7222 mL	13.6110 mL
20 mM	0.1361 mL	0.6805 mL	1.3611 mL	6.8055 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc